It is my first research (Thank should be done by me from A-Z ) and I want to know the new technologies and techniques that used to transfer the histological findings to bio statistical numbers.
you can use eyepiece micrometer (traditional method) for different histological measurements.
but software like Image J is better, by using this program u can take all measurements from the photo, it is more precise and repeatable.
Md Abdullah Al Mahmud and Eman Eshra
I cant thank you enough Bro.
Jazakm Allah Kheer All
Try ImagJ dear Mohammad. it is a user friendly software.
Dear brother Mazaher Gholipourmalekabadi
Thank you so much. I downloaded it and started using it.
This is my first real research thank you for being with me helping and guiding since the beginning.
Dear Mohamed,
I do recommend ImageJ. You can count cells, measure distances and also measure fluorescence intensity. What kind of tissue or organism are you interested in?
If you are interested in ImageJ I do recommend you read the attached file. It describe several steps for beginners.
Cheers,
João
The data that you can get from histological slides to analyze statistically can be one of three types:
1. Nominal (categorical - binary): for example, stained or unstained, damaged or normal, present or absent ... etc i.e, either this or that.
2. Ordinal: which has levels or orders, e.g, mild, moderate or severe -
3. Scale (numerical): e.g., number of cells, distance, angle, perimeter, surface area, density ... etc. Only, for this type of data you need an image analysis software such as image J or FIJI.
After you decide which data you are going to measure, you have to decide what is the question of your study? so that you can choose the proper statistical test.
1. If you want to make sure that the differences between the study groups didn't happen by mere chance, then you measure probability by comparing means to find statistical significance (such as t test, ANOVA, Chi square etc.).
2. If you need to find if two groups are correlated or follow a similar pattern then you do correlation analysis.
3. If you suspect a predictive role of one group on the other then you do regression analysis (e.g, if one group increases, the other also increases)
Dear Professor Hany Shawky Nadim
I cant thank you enough you just summarized a book.
Thank you so much.
Dear Dr. Darvish. How are you. It's 15 years already. I'm glad to hear from you
hello Dr. Nadim
hope you and your family are doing well and staying safe.
by the way it’s been 25 years!
take care,
Ahmadp
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