High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

Does anyone know what is chemical structure of ALC-0307 lipid?

I tried to contact Acuitas Therapeutics regard their patented lipid ALC-0307 but heard nothing back. Some lipid supplier such Avanti also helped me to contact Acuitas but still didn't get any...

09 May 2023 3,589 0 View

How to enhance the ionization of liraglutide during mass spectroscopy?

At the beginning, my method was good and I was able to analyze Liraglutide effectively (peak area of Liraglutide 500 ng/mL was 2364616). But, these days, I have been facing problem of decrease in...

07 May 2023 4,291 1 View

How to use Discovery Studio Visualizer (DSV) for generating a list of protein-ligand interactions automatically?

I have to make tables including the data of the nonbnding interactions (such as type of interaction, distance...) of protein ligand complexes. Usually, I use DSV for this kind of analysis, but I...

07 May 2023 766 0 View

What is the dichotomy of desk review and Literature review?

I need clear differences between the above two reviews.

06 May 2023 1,400 3 View

Please can someone share the sample preparation procedure for ICP analysis of biochar?

Testing for metal content in biochar

04 May 2023 5,663 6 View

How to filter protein sequences (from Uniprot or Interpro) using databases such as Representative Proteomes or Reference Proteomes?

When downloading protein family alignment from very large families, I usually chose the filtered options from Pfam (Representative Proteomes or Reference Proteomes) in order to have a manageable...

03 May 2023 8,453 2 View

Anyone have knowledge of synthesis of peptide named argireline by SPSS totally? And ways to avoid +56(might be +tbu) impurity formation?

We are facing problem while doing cleavage. As we get final crude after using many combination of TIS Water & TFA and other was TFA TIS Phenol & DTT still not getting good results as...

03 May 2023 2,808 2 View

Can anyone help me in making synthetic recipe of petrochemical industry wastewater?

The COD of real wastewater is 400 ppm and TDS is 700 ppm. Oil and Grease content is 30ppm and BOD is 200 ppm.

03 May 2023 2,974 1 View

Does anybody know an R package that can do simultaneous global fitting based on 8 curves?

I basically have normalized fluorescence data generated from a series of protein dilutions. I need to calculate K, K should be the same in all dilutions, thus the need for a global fitting...

29 April 2023 2,387 2 View

How to synthesize a peptide with 3 disulfide bridges?

Dear colleagues, Can we synthesize Alpha-Defensin-4 (Human; 33-mer) peptide with 3 disulfide bonds between Cys2-Cys30, Cys4-Cys19 and Cys9-Cys29 using the Microwave Liberty Blue peptide...

28 April 2023 532 0 View

How to extract 10-HDA from Royal Jelly in order to further use it in experiments?

Hello! I am looking for an isolation and purification protocol for 10-HDA (Royal Jelly’s main fatty acid). I have searched the internet, but did not find any possible method to extract this...

28 April 2023 9,047 1 View

Is it ok to store a protein A chromatography column in 20% EtOH with no NaCl, or will this damage the resin?

N/A

28 April 2023 212 1 View

Is there a simple test method for the online determination of dissolved gases in water (Especially conductometry)?

I plan to introduce the chlorine gas resulting from PVC degradation into the water. How can I measure online the amount of chlorine that will be injected into the water?

27 April 2023 7,928 8 View

Can someone help me with synthesis of Fe3O4-Urea nanocomposite ?

I am planning to attach urea to the fe3o4 nanoparticle. I have already gone through a few papers and tried the sonication method to attach but was not successful. I will be pleased if someone can...

24 April 2023 9,365 4 View

How to improve mobile phase mixing in a quaternary pump?

In order to analyze small-MW thiol metabolites in plasma, I typically use a method with a mobile phase made of acetate buffer 0.1M (pH 5.25; 96.5%) and methanol (3.5%) running at 0.6 ml/min on a...

17 April 2023 5,223 14 View

What is the shelf life of organic solvents used for LC-MS? Is there any valid data to substantiate device manufacturers recommendations?

Does anyone know any official document or scientific publication logically explaining why I should replace for example HPLC-grade acetonitrile or methanol (pure, or with

15 April 2023 5,773 4 View

How do you make a 1M triethyammonium acetate solution with the correct pH?

I am trying to make 1M triethylammonium acetate with triethylamine solution and glacial acetic acid solution. I am mixing them in the recommended volumes to yield a 1M solution (6.97mL...

14 April 2023 4,801 1 View

How to solve the precipitate in the total phenol test and HPLC of NADES(natural deep eutectic solvents)?

In the total phenol test , when I added forinphenol reagent into NADES, my 96-well plate will produce yellow precipitate, resulting in the inability to detect absorbance.But I noticed the others...

12 April 2023 9,996 0 View

If there's a complex ion containing two different ligands, is it possible to substitute a selective ligand?If so, then how?

I work on Prussian blue dye which has cyanide ligand and coordinated water in it. I want to replace the coordinated water with some other strong ligand

10 April 2023 5,735 0 View

What about the studies that are commonly used in the precipitation technique of heavy metals removal?

In the adsorption removal of heavy metals, we typically focus on kinetics, thermodynamics, and isotherm. What about the studies that are commonly used in the precipitation technique?

09 April 2023 412 5 View

What is the best method for bacterial disinfection of drinking water and why and effective method for killing microorganisms in contaminated water?

03 April 2023 7,546 7 View

Is it okay to add molecular reactivity calculation based on quantum mechanics (DFT HOMO LUMO) in an ethnobotany paper??

In our recent work we have used FIC values to find the best species against a particular disease. We have docked the bioactive phytochemicals of that particular plant against specific receptor of...

02 April 2023 2,311 2 View

Why the TOC concentration of Rhodamine B increased after photodegradation?

The photodegradation experiment materials included RhB, catalyst (catalyst does not include carbon or carbon compounds), crystallizing dish, and magnetic stirrer. After 2 hours of irradiation, the...

29 March 2023 7,269 9 View

How to make nanoparticles inhibition bacteria sample for SEM image?

I want to get some images of how nanoparticles work in bacteria cells. I used scanning electron microscopy. how to make samples, and how much use bacteria concentrations?

29 March 2023 8,890 3 View

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