Dear all
I am trying to do an electrochemical detection of cholesterol and triglyceride, so kindly help me with some valuable inputs. Kindly help me with some reference for the same .
Thank You In Advance
For cholesterol: You have to add surfactant like Triton X-100. For e.g if you want to prepare 30 mg/ml stock solution, then add 60 mg of cholesterol to 1 ml of Triton X-100 and stir it under heating at ~ 90-95 deg C until the solution become transparent. Then add 1 ml of IPA, allow it to stir for 2 min, bring to room temperature. You can store it in 4 deg C and prepare the dilutions in whichever solvent you want when DI water.
For triglycerides: If you are using triolein, mix it first with Triton X-100 and then with Tris-HCl buffer. heating is not needed. This solution will be milky but homogeneous. Prepare the dilutions in water or PBS whichever solvent is needed.
Hope this helps
- Badges
- Science method