High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

Why researchers use different doses of infection for experimental trial for a same kind of disease model?

I see many papers that used different doses of Salmonella Enteritidis in the chicken infection model for the same type of chickens e.g., Ross-308 broiler. It also varies at different ages of the...

23 October 2023 7,706 1 View

Any idea how I can find protein domains on BLAST?

Hey guys, I'm doing some research on cadherin proteins. How can I find all human proteins with a cadherin domain using BLAST or biopython? Any ideas? Thanks

21 October 2023 3,121 1 View

Comparing moving range standard deviation in control charts?

To see if the improvement was effective, a process with before and after measurements are plotted in control chart. Is it possible to test the control limits and say if it is significant.? I am...

19 October 2023 2,101 1 View

Quinolinic acid standard curve show reverse linearity when mixed with Blank serum samples?

When i use quinolinic acid in inreasing concentrations along with blank serum, the standard curve is surpisingly negatively linear! Expected linearity is reverse for this. However other standards...

19 October 2023 5,635 0 View

How can I upload my research paper to Research Gate ?

I have my work on a particular topic and I would like to share it with Research Gate. Show me please, how to do it.

18 October 2023 2,159 4 View

How to find the probability of binding of the mutant transcription facor to the promotor of the desired gene?

Hello, We have a gene that is modulated by the binding some transcription facors (TFs). We recently found that one of these TFs is mutated and we want to know if this TF can still bind to that...

17 October 2023 6,677 3 View

Is biodesalination a viable solution to global water scarcity in the future?

Biodesalination is a concept that combines bioremediation and desalination processes to address water scarcity issues by removing salts and other contaminants from brackish or seawater....

12 October 2023 1,579 2 View

We have run SDS PAGE, but it is moving irregularly What would be the possible reason for this?

What would be the possible reason for this: Following are our PAGE setup; Protein Source:Rat Liver Preparation : Ground in Lysis buffer and centrifuged and collected supernatant. Equalised all...

11 October 2023 2,194 5 View

Are there alternatives to Neurolucida to trace stain from confocal images stacks?

We are seeking to count cell death stain from confocal image stacks; however, Neurolucida is quit expensive. Are there any alternatives that you recommend? Is ImageJ viable?

09 October 2023 1,239 1 View

How to solve the error in gromacs "Warning: Only triclinic boxes with the first vector parallel to the x-axis are supported" ?

Hello, I am getting the error below almost at the end of the simulation performed using gromacs 2023.1 . I am sure I did not encounter this problem before with the same input files. This has just...

09 October 2023 5,405 0 View

How to recrystallization of aluminium from sodium aluminate ?

I used sodium aluminate, Can i need re crystal of Aluminium for zeolite synthesis? How this process and which is best solvent? How can i understand this is perfectly transform pure aluminium ?

08 October 2023 814 1 View

What other process do you think can help remove impurities from methanol and what kind of impurity can I have?

HPLC grad methanol was contaminated with industrial methanol. In order to remove the impurities created by industrial methanol, the distillation process was done with a Vigreux column, but...

05 October 2023 1,085 0 View

Please advise if there is any effect to adding black soldier fly powder (BSFL) on broiler meat Sensory?

please advise if there is any effect to adding black soldier fly powder (BSFL) on broiler meat Sensory?

04 October 2023 1,730 6 View

Hi! How do you combine 2 drugs to study synergism?

I am going to combine 2 drugs at a constant ratio and perform MTT. I usually use 100 uL of drug on each well. Should I do the solutions of the drugs separately and add 50 uL of each drug to the...

03 October 2023 933 0 View

Need to have a research article reference of application areas of graphene that has been done in the year between 2021 to 2023?

Recent progress in graphene research and feasibility of various graphene application in the year between 2021 to 2023

29 September 2023 7,774 3 View

Dissolution testing for oral pharmaceutical products - QC and biowaiver methods?

Hello, Could anyone direct me to a consultant specialized on developing disso method for QC and IVIV biowaiver please? Does anyone could direct me to disso training covering these two topics...

29 September 2023 2,967 1 View

Dear all , I am working on nanoparticles and their Biological applications. I want to check the bacterial proteins and DNA damage of Nanoparticles?

I am working on nanoparticles and their Biological applications. I want to check the bacterial proteins and DNA damage of Nanoparticles?

26 September 2023 4,266 1 View

EP cannot be found when titrating acid soluble chloride from concrete with potentiometric indication ?

Dear Researchers Kindly comment on the problem I am facing during titration. To quantify chlorides ion in concrete, I prepared the sample following ASTM C1152. The chlorides were extracted from...

25 September 2023 3,248 1 View

Is it possible to perform correlation or regression analysis between two scales with different ratings (5-point Likert and 7-point Likert)?

I need to test the relationship between two different variables. One of the variables is calculated as 1-5 points, the other as 1-7 points. Does having different scale scores cause an error in the...

25 September 2023 4,855 7 View

What is the solubility of pure glycine hydrochloride in pure water?

I would have thought that the solubility of glycine hydrochloride (not that of the zwitterion, nor that of the zwitterion adjusted to low pH with HCl!) would have been measured and published long...

23 September 2023 6,077 0 View

Are you concerned about the impact of AI in advancing education in schools?

AI Education Impact

22 September 2023 1,904 9 View

I did quantification of drugs along with their process related impurities. Can this be compared with the assay methods reported in literature?

I am a Ph.D research scholar in the department of pharmaceutical analysis.

19 September 2023 5,141 4 View

Has anyone tried dissolving PVA (poly vinyl alcohol) in water?

I am trying to dissolve PVA in water and even after boiling it does not dissolve. Is there any specific method to dissolve it in water? I am trying to make a 10% PVA solution in water.

15 September 2023 3,085 0 View

Do women understand men?

Necesitamos discusiones al respecto, ante la tendencia de cuestionarse la capacidad de los hombres para entender a las mujeres y viceversa.

14 September 2023 3,557 1 View

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