Hello to all,
I am working on SH-SY5Y cells and I treated my cells with Rsl3 and ferostatin-1 (24 hours) but I could not see good cell viability. I would like to know how I can treat my cell so that fer-1 can prevent cell death caused by Rsl3.
With thanks for your attention
Parisa
Hi Parisa Faraji
Firstly, I'd suggest ensuring that you're using appropriate concentrations of both RSL3 and Ferrostatin-1. The concentrations required can vary depending on the specifics of your experimental setup, including cell density and the duration of exposure. In many cases, concentrations are determined through a process of titration, starting at lower concentrations and slowly increasing until the desired effect is achieved.
Here's a general process to follow:
Remember to keep in mind the half-life of Fer-1, which is approximately 2 hours in cell culture medium, so repeated administration may be necessary for longer experiments.
Monitor cell viability using an appropriate assay such as trypan blue exclusion or MTT, and adjust concentrations as necessary. Note that while Fer-1 can prevent RSL3-induced cell death, it may not restore cell viability to 100%, as some cell death may still occur.
Hello Parisa Faraji
I asked my colleague from college, and he suggested that " She can try to optimize the concentration, First use cell with only fer-1 with different concentration. And then see the effect of cells treated with different concentration of Rsl3. Then see the combination according to the previous observations, it's usually a concentration problem, or she can treat the cells with fer-1 for 2 hrs then add Rsl3. She should read the literature carefully. Maybe he can find some clues about Rsl3 minimum concentration toxic effect.
I hope you may get something from his explanation. All the best.
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