How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

How to define thermal failure in Abaqus?

Hi, everyone, I am simulate the impacting CFRP model, I want to know how to define thermal failure during the impact process because epoxy degraded owing to thermal effects. Thank you so much for...

25 December 2020 7,424 3 View

How to define thermal conductance in Abqsus?

25 December 2020 3,596 1 View

ESA Eureca spacecraft Solar Array Arcing demage, What is the cause of the problem?

We see a photos of solar array damage suffered by the ESA Eureca spacecraft that was recovered by the Space Shuttle. photo shows a sustained arc site on its solar arrays. In this case, the...

23 December 2020 2,512 2 View

Micro-scale unit in Abaqus?

Micro-scale unit in Abaqus. Hi everyone, I am working on micro-scale simulations, the length of the model is micrometers. So, some information is provided on the internet, but some part is...

10 December 2020 8,448 3 View

How to reduce background noise when testing cholesterol level in rat serum?

l've got rat serum samples with different background (from pink to yellow) and l'm trying to test total cholesterol, HDL and triglyceride in mice serum. l'm worried that the background noise...

14 November 2020 6,628 3 View

Is Uniform Convergence property contradictory to No Free Lunch Theorem?

In Statistical Learning Theory, Uniform convergence means for a hypothesis class H, given m(\delta, \epsilon), for arbitrary distribution over domain (X, Y), with probability $1- \delta$, the...

03 November 2020 7,732 5 View

How to recognize and verify the crystallization occur or not?

Hi, everyone My feedstock powder is water atomized Sn with a diameter range from 10-100 um. After the cold spray processing, the EBSD maps can be obtained. Because my smallest powder is 10um,...

07 September 2020 7,766 4 View

What is we need for Sequence learning (Speech, NLP, Finance, Transportation and so on)?

In 2017, a famous paper proposes Transformer which successfully adopt self attention as well as FFN for NLP. The paper is called "Attention is All You Need". This work lets many scholars know RNN...

11 August 2020 8,119 11 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View