Why my SDS-PAGE bands turn out like dumbbell-shaped and funnelform?

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Venketsubbu Ramasubbu

Huihuang Sun i am sure your gel components has gone bad as i see the lower band and check the concentration of the protein you load

Usha Kantiwal

It seems like the concentration of protein samples loaded is too high. You can try with 1/2, 1/4, and 1/8 sample volume per well (less protein/well). I'm sure the picture will be more clear. Wish you the best!

Huihuang Sun

Usha Kantiwal thanks for you input, I am so appreciated.

Venketsubbu Ramasubbu thank you so much, extremely thank you for answer