Hi does anyone here experience missing formation of uroporphyrin and heptacarboxyporphyrin peaks? I do analytical test using chromsystems kit. Only this year i attempted the column was unable to achieve more than 50 injections. I lost the peak of uro and hepta and i dont know what might be the culprit. Could it be the column? The column is RP C18. Here i attach images. Really need someone who experienced the same

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