High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

How to synthesize N-Cbz-protected peptide by Fmoc/tBu-SPPS?

Dear Colleagues, How can I synthesize a peptide with N-terminal protection by the benzyloxycarbonyl group (Z-group) using Fmoc/tBu Solid-Phase Peptide Synthesis? Can Z-Leu-OH be coupled during...

09 June 2023 3,376 1 View

Who holds copyright to original drawing made by purchased software?

In the course of preparing a journal/book publication based on my original research, I prepare a set of drawings using commercial software designed for scientific applications. Am I the copyright...

09 June 2023 7,031 0 View

Why is there a difference of 1Da in calculated vs experimental mass spectrum?

Hello, I'm working with highly charged supramolecular systems and there is a discrepancy of one unit between my experimental and calculated data. I'm using ChemCalc to calculate the mass spectrum...

08 June 2023 7,796 0 View

Troubleshooting: Gel Solidification Issue in SDS-PAGE Electrophoresis?

Dear ResearchGate community, I am currently facing an issue with the solidification of my SDS-PAGE electrophoresis gels. Despite following the standard protocol, the gels fail to solidify...

07 June 2023 9,984 1 View

How to calculate Rsh and Rs of a solar cell?

How to calculate Rsh and Rs using the manufacturer's datasheet and if the I-V curves are not provided? I am unable to find the slope as i do not have enough values.

06 June 2023 9,126 2 View

About mouse IL-6 Elisa assay from R&D system, what's the dilution of mouse serum sample?

Hi, I have a question about mouse IL-6 Elisa assay. I am preparing for this Elisa assay, but I am not sure how much dilution that I should do for this assay. My sample is serum from mouse....

05 June 2023 9,692 1 View

Can anyone have a good protocol for isolation of CD138+ plasma cells from human bone marrow samples using FACS?

I am going to work with multiple myeloma bone marrow samples. I need to isolate the CD138+ plasma cells. The sample will be processed the next day due to logistic reasons. Can anyone share their...

02 June 2023 10,003 0 View

Need help to get Alpha EaseFc software to quantitate western blot?

Hello All, I am in need of Alpha EaseFC software to quantitate Immunoblot protein bands. I know other options (such as ImageJ) are available to for this purpose, but I am specifically looking for...

30 May 2023 2,908 1 View

Can nontoxigenic strain of A. flavus cause aspergillosis ?

biological control of aflatoxin is very popular these days,I just want to know if nontoxigenic strains can lead to infection like aspergillosis, if yes then why we are using these strains for...

28 May 2023 8,001 2 View

How to obtain a calibration curve for an antibiotic?

I'm currently working on the degradation of an antibiotic (penicillin). To achieve that, I need a calibration curve. I tried several times but each time I can't get the appropriate absorbance for...

26 May 2023 9,580 4 View

Is there any correlation between salt concentration and lost protein during concentration?

Hi. I am a student working on a research project aimed to express a protein, purify it and concentrate it. To concentrate my proteins, I am using a centrifugal concentrator (10K MWCO). I noticed...

25 May 2023 3,954 1 View

How to conserve seeds?

Greetings, I have dry seeds, and I'm looking for a good way to store them and keep them in good condition, with no contamination or appearance of insects. Thank you.

25 May 2023 258 5 View

How can I do anti-inflammatory assay by plate reader?

Dear friends, I am trying to measure anti-inflammatory of chamomile according modified Williams et al. method by BSA at 660 nm. The problem is that data is over even empty plate is approximately...

23 May 2023 6,721 0 View

Why do people etch a part of the ITO layer away before fabricating a regular perovskite solar cell?

Hello. What is the reason behind the partial etching of ITO for perovskite solar cells? I know it should have anything to do with the measurement of the J-V characteristics. Thank you for...

22 May 2023 1,606 0 View

Why we have on signal for Lockspray in SYNAPT G2 UPLC-HDX Waters?

We see no signal for Lu-Enk when we inject it through Lockspray line but when inject directly from the sample line, we see the signal. I have to say that No high pressure is observed. How can I...

22 May 2023 3,851 0 View

Which temperature is best for drying Carboxy cellulose nano fiber on Vacuum oven drying or freeze drying? and need to pH 7.00 adjust before drying?

I,m synthesis carboxy cellulose nano fiber but gradually material's pH going to acidic without centrifuge. now my step is drying this material's. how can i do this?

21 May 2023 3,195 1 View

Would unsaturation or addition of a carbonyl group on the alkyl chain of phospholipids change their solubility in MeOD?

solubility issues in NMR samples

21 May 2023 6,625 1 View

Can anyone share freely accessible tools to make scientific diagrams/figures for publishing in reserach articles?

Though plenty are available, can anyone suggest user friendly tools for making scientific figures, tables and diagrams that are avilable free of cost?I am trying to use Create flowchart, Mind the...

18 May 2023 8,399 0 View

Extraction solvent of saponins from oily seeds?

Hi all I am currently embarking a project to determine saponin content in oily seeds, I have been looking at various literature methodology and according to available data in the literature,...

16 May 2023 1,219 1 View

AKTA Pure pressure with no column?

Dear all, I am running some gel filtration FPLC analysis in an AKTA Pure system. There's an issue, the pressure in the system with no column attached is already ~0.3 MPa, which is very high...

15 May 2023 9,385 2 View

Why few number of cells are expressing transgene on application of Dox despite the cell line being generated from single cell cloning?

I've generated a Dox-inducible transgene overexpressing cell line after lentiviral transduction followed by single-cell cloning. The problem I am facing is that only 20-30% of the cells express...

15 May 2023 9,383 1 View

How can I percipitate short peptides after resin cleavage from Rink Amide?

I am trying to separate a small peptide (4 amino acids) synthesized using solid-phase synthesis after cleavage. I have tried dissolving the peptide in diethyl ether, but it did not percipitate...

11 May 2023 7,598 3 View

Why is the Lab button not there to the right-hand side of my Profile to start a Lab?

Following your instructions, how to start a Lab https://help.researchgate.net/hc/en-us/articles/14293170669713-How-to-create-and-edit-a-Lab I am not able to find the Lab section on the...

11 May 2023 6,183 0 View

Which material can replace PEDOT:PSS in QLEDs??

which material can replace PEDOT:PSS in QLEDs serve as Hole injection layer??

10 May 2023 1,781 1 View

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