High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

How to get mycelium of Trichoderma in culture media?

Currently working on trichoderma spp., but getting only spores in media. How to grow and observe trichoderma mycelium in PDA or other culture media. Kindly help.

15 December 2023 8,239 2 View

Thermodynamic data on the formation of rhamnolipids?

I am trying t understand the thermodynamics of bio surfactants, particularly Rhamnolipids. All I find is information on the free enrgy of formation of micelles, which is not of interst for my...

15 December 2023 9,044 0 View

KingFisherDuo -elution volume- evaporation?

Hi! wonder if anyone has experience performing RNA extractions with the KingFisherDuo platform and what happens with the final elution volume. Has anyone experimented loss of final volume? ie from...

13 December 2023 6,122 0 View

How to dissolve the ceramides and add it to cell culture for treatment?

Dear all, I recently encountered a problem with how to add exogenous ceramide to cell culture to monitor their biological effects. Currently, we want to add two ceramides to the cell culture：...

12 December 2023 7,689 3 View

Why is Ni3+ not as common as Fe3+?

I have been pondering why nickel only exists as the Ni2+ ion in nature. I know that Ni+ and Ni3+ also exist, but unlike iron (Fe), Ni3+ is not nearly as common as Ni2+. So, I have investigated...

11 December 2023 3,274 2 View

How long does it take for the hCMEC/D3 cell to acquire its spindle-shaped structure after thawing?

Hello, even though 7 days have passed since we thawed the newly ordered cells, they are still in a round shape structure. We are using Lonza EBM-2 and we supplemented the media with: Fetal Bovine...

11 December 2023 8,637 0 View

How to calculate the organic cation radii?

What methods or software can we use to calculate the ionic radius of the organic cation?

11 December 2023 559 0 View

Can a randles-sevcik equation be used for a quasireversible reaction?

In my experiment, the peak-to-peak separation does not fall under the conventional value of 57 at 25 degree Celsius due to a temperature change. I am assuming that the reaction may be...

09 December 2023 1,847 0 View

Is it possible that amyloid formation of a proteins doesn't always happen even though I did the same process for replications?

Is it possible that amyloid formation of a proteins doesn't always happen even though I did the same process for replications? Sometimes they aggregate and form amyloid however sometimes there is...

08 December 2023 4,093 3 View

How to lyophilize of EP Fungi?

I need to lyophilize Fungi that Has been Ethanol Precipitated and dialyzed in water. I am having problems, because it unfreezes (after being frozen in liquid nitrogen) after I connected to the...

08 December 2023 6,053 2 View

How to Prove that the change in energy form is an adaptive behavior to the surrounding environment and not a forced one?

How to Prove that the change in energy form is an adaptive behavior to the surrounding environment and not a forced one. I think there's a possible reaction mode to prove. But I need a exist...

06 December 2023 4,240 0 View

How to design vector for overexpression of a heterodimer protein ?

human Topoisomerase is a HOMODIMER Protein (AA). Now i want to mutate one chain to produce a heterodimer version (AA*). but there are chances of production of three products (AA, AA*, A*A*). So...

05 December 2023 8,828 1 View

Whether it's possible to create a TIC (Total Ion Chromatogram) using only specific phytocompounds from High-Resolution Mass Spectrometry (HRMD) data?

05 December 2023 4,109 1 View

Help for Adsorption MB calculation-C equilibrium?

Hellow Professors. i am doing adsorption of methylene blue experiment. i monitored with uv visible spectrometer by taking 10-100ppm solution with time up to 40 min(10 min interval). but I unable...

03 December 2023 4,341 3 View

What can i do with precipitated lignin from biomass pretreatment liquor?

What is the next step i can move on to with this material to create some value-added product

02 December 2023 2,147 5 View

HEK293T cells culture?

Hello, i have grown my HEK293T cells in 4 wells of a 24-well plate and added different serial dilutions of lentivirus(containing my gene and GFP), now i want to perform FACS analysis so for this...

01 December 2023 8,827 2 View

How to analyze enzyme activity when given different units and no definition of U?

I am currently trying to compile a meta analysis looking at the influence of enzyme activity of xylanase on fiber digestibility. However, much of the research I have compiled have different...

28 November 2023 7,895 3 View

Hello! I am working GWAS. I am facing a problem while performing the GLM model. Thus, may I have solution from the specialist?

myY

28 November 2023 938 0 View

Remove the magnetism of mechanism arrows in ChemDraw / Free angles to draw arrows in ChemDraw?

Dear all, This has been a long time wondering how to handle arrows in ChemDraw, especially for writing mechanisms. When choosing the arrow tool, the angle is not human-chosen and unfriendly,...

25 November 2023 7,367 0 View

How to settle the circumstance of molecular docking when charge=0 ?

And I was using imidacloprid for molecular docking. When setting the ligand, the charge of imidacloprid was always calculated to be 0, and the docking reality was wrong.

25 November 2023 1,541 1 View

Unusual case during stability study in ADC formulation? Contamination or protein degradation?

Hi all, For context, I am conducting a stability study using an ADC material formulated in different buffer systems to screen for the optimal pH/buffer to use for the final drug formulation. The...

24 November 2023 5,452 2 View

What is the role of microorganisms in soil fertility nitrogen cycle and effect of denitrifying bacteria on soil fertility?

22 November 2023 6,153 5 View

Can anyone suggest an optimized protocol for collagen degradation/digestion using collagenase enzyme?

I need information on concentration of enzyme to be used for particular amount of collagen, the buffer solutions, temperature, time, pH required for the optimal enzyme activity. According to the...

20 November 2023 6,428 1 View

Why is the limit of enalapril impurity D (diketopiperazine) higher than the qualification threshold in ICH Q3B?

In British Pharmacopoeia, the limit of Enalapril impurity D is 1%. However, in ICH has specified that with Maximum Daily Dose 10-100mg (Enalapril Maleat is 40mg), Qualification Threshold would be...

19 November 2023 1,194 1 View

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