High-Performance Liquid Chromatography

High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) is a chromatographic technique used to... | Contact experts in High-Performance Liquid Chromatography to get answers

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Questions related to High-Performance Liquid Chromatography

Is it mandatory to maintain inert atmosphere for Poly3-hexylthiophene (P3HT) synthesis through oxidative coupling using ferric chloride as catalyst ?

I am synthesizing P3HT by oxidative polymerization of 3-Hexylthiophene by using FeCl3 as catalyst. While going through the relevant literature, I came to know that polymerization has to be...

13 September 2023 4,932 1 View

Can we induce DNA damage in cancer cell line using a UV light in the laminar flow hood?

Hi, I want to induce DNA damage using the UV light in the laminar flow hood? I inquire is that possible and what is the required duration? Thanks,

13 September 2023 3,019 1 View

Colony PCR - results explained?

Hello, Can anyone help me understand what my results are showing. My aim was to express the SucC gene in pET15B from pET3a-SucC. I performed a colony PCR with 4 ligation tubes and 1 control and...

12 September 2023 6,066 2 View

How to draw tafel plots for OER and HER?

I need to know the exact method of plotting tafel slopes for OER and HER. Thankyou

12 September 2023 2,249 5 View

Why is winter the coldest season because the sun's angle is making the sunlight weak and relationship between population increase and climate change?

Why is winter the coldest season because the sun's angle is making the sunlight weak andrelationship between population increase and climate change?

11 September 2023 4,676 5 View

After testing my research hypothesis, what is the next action to take?

I have performed a hypothesis testing using the simple regression analysis model. What action must I take after testing the hypothesis?

11 September 2023 2,466 7 View

What are the effects of salinity on germination and growth of radish seeds?

The different effects of different concentrations of salt in water and how they may affect germination and growth of plant seeds. The sources of info that can offer more details on conducted...

09 September 2023 2,282 1 View

What could cause more formazan crystals than cells could convert from MTT?

Hi everyone, I'm having some trouble with classical MTT assay. when taking OD at 570nm I'm getting invalid values like 6.000. what causes more formazan crystal formation in the well than the cells...

08 September 2023 2,088 5 View

PREDICTION PROBABILITY in Logistic regression?

I am using SPSS to perform binary logistic regression. One of the parameters generated is the prediction probability. Is there a simple mathematical formula that could be used to calculate it...

07 September 2023 941 5 View

Why is it P(|T|>=t)?

The p-value from the T-test is denoted as P(|T|>=t). Yet, observing a t-statistic similair or more extreme then T under H0 based on what I know does not fit with this notation. Often a small...

06 September 2023 9,900 3 View

Mannan and glucan detection?

We are working on a quantification method for mannan and glucan detection by spectrophotometry. Glucan and mannan are separated during the extraction process, however, we cannot calculate its...

04 September 2023 4,333 1 View

UVWin Lab software- Perkinelmer?

Hi everyone, Does anyone know about putting conditional formulations as IF in excel, into UVWin Lab software of Perkinelmer Lambda 365? Thanks.

03 September 2023 5,628 0 View

¿Does a classification or taxonomy of adaptive control exist?

Hello: I have a question about the definition of adaptive control, since I´m researching about making a model-free adaptive control system. I will appreciate your help. The definition I found...

01 September 2023 9,692 3 View

Histidine-Tag location?

Have any of you used the Nzytech expression system, especially the pHTP8 expression vector? I have some doubts about the site where the histidine tag is located.

31 August 2023 4,178 2 View

Which labelling agent is recommended for PNA (peptide nucleic acids) in gel thermophoresis?

My experiments include ssDNA, dsDNA, and DNA-PNA hybrids

31 August 2023 1,679 0 View

Can citric acid solution dissolve Fe3O4?

Can citric acid solution dissolve Fe3O4? What is the best solution for dissolving Fe3O4?

29 August 2023 6,652 2 View

How to adjust C/N ratio in Lignocellulose sugar fermentation if Urea is used for pretreatment?

I'm using Urea for pretreating Lignocellulose materials particularly Sugarcane Bagasse, How to adjust C/N ratio and what is recommended ratio for fermentation using S. Cerevisiae ? My fermentation...

28 August 2023 2,515 2 View

How to Distinguish between dh5 alpha and bl21 cells ?

How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?

26 August 2023 6,973 0 View

What is the best statistical analysis to use on SPSS? 3 DV and 1 IV ?

Hi all, please assist me on what statistical analysis I should use. I have 3 DVs (severity of violence, justification of violence and severity of punishment) and 1 IV (gender of perpetrator). I...

22 August 2023 5,291 3 View

What affects the direction in which roots grow and what directs root growth toward gravity and stem growth in opposite direction?

22 August 2023 5,064 0 View

How can I make sure that the process is correct for making cocrystal design or not?

1. firstly, we need to create the conformation of our target molecule. mercury>CSD-materials>conformer generation 2. secondly, need to design...

22 August 2023 8,059 1 View

How to make cholesterol and triglyceride stock solution ? In which solvent they are soluble?

Dear all I am trying to do an electrochemical detection of cholesterol and triglyceride, so kindly help me with some valuable inputs. Kindly help me with some reference for the same . Thank You In...

19 August 2023 8,602 2 View

Where can one get the global statistics on CO2 reinjection for enhanced oil recovery and storage purposes ?

I am working of the impediments of Co2 reinjection and would like to review the current reinjection statistics.

14 August 2023 5,487 2 View

How to determine the number of broiler in feeding trial?

I need to conduct a feeding trial on the broiler, 5 dietary treatments 3 replications. How many broilers should be placed totally and in each single treatment?

10 August 2023 9,861 3 View

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