Estimation of total polyphenolics
The reaction mixture contain 50 μl of sample, 3 ml water, 0.25 ml FC reagent and 0.75 ml 20 % Na2CO3. The total volume make up to 5 ml using water. Mixed well and incubated the mixture at 50°C for 2 hours. Read the absorbance at 765 nm using spectrophotometer [23]. Here gallic acid was used as the standard. The concentration of total polyphenolic content was obtained from gallic acid standard curve by using the following formula.
T = C × V/M
T = Total polyphenolic content expressed as mg/g of the sample extract in GAE
C = Concentration of Gallic acid from caliberation curve (mg/ml)
V = Volume of extract (ml)
M = Weight of sample extract (g)
The amount of total phenol in plants
The equivalent form of milligrams of gallic acid (GAE) per gram of weight
It was calculated dry.The amount of total phenol in plants
The equivalent form of milligrams of gallic acid (GAE) per gram of weight
Dry is calculated.
The measurement of total phenol is done by Folin-Ciocalteu method.
Jenson Jacob Thank you very much for your answer. Can you please provide the reference?
The amount of total phenol content (TPC) of plant extract measure according to the method decribed by Taga et al. (1984). In this method, 100 μl of the extract sample mix with 2 ml of 2% Na2Co3 and place at room temperature for 2 minutes. Then, 100 μl of 50% Folin Siocalto reagent add, mix, and place in the dark for 30 minutes at room temperature. Samples absorption measure at 720 nm using an Elisa Reader device. The results express as µg gallic acid per gram dry weight.
Reference: Taga, M.S., Miller, E.E., and Pratt, D.E. 1984. Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists’ Society, 61: 928-931.
Torres AM, Mau Lastovicka T, Rezaaiyan R. Total phenolics and
high performance liquid chromatography of phenolic acids of
avocado. J Agric Food Chem 1987;35:921-5.
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