Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?
most of expression biomarkers at the RNA level appear to be extremely sensitive to variety of everyday exposures: food additives, drugs, flue, etc. I am not sure, that normalized expression is an adequate signature. Best way (in my opinion) is to use ddCt within a single subject/person/patient.
As per my knowledge of the NanoString PAN Cancer gene panel, The NanoString analysis is specifically carried out on its own nCounter software which generates three files (1) Raw data (2) Normalised data (3) Ratio data.
Now from the Ratio data, those DE call is 'YES', are only being considered. Ratio data is generated from Normalised data. So, Normalised data won't be the one for representation. What I am saying its associated with gene expression.
miRNA count which you mentioned, maybe different or more or less same.
So, better consult the Nanostring xpert, they responds quickly in chat or mail.