I am going to inhibit Hela cell growth to see whether it can change one gene's expression?
Thank you!
hi,
Currently, a lot of established and potential cell cycle inhibitors are available in the field. So, you can choose them according to your needs such as target, mode of action, checkpoints, etc.
Now it is very unsure to say that which inhibitor will be less toxic. So, you have to find the LC50 concentration of inhibitors using cytotoxicity assays against your cell of interest. Moreover, it's not just toxicity, concentration should be effective to cause inhibition followed by a change in gene expression. The concentrations of inhibitor not necessary that it will have a concentration-dependent effect, slightly less or high concentration may give a different result, you have to find that particular window because that concentration should not affect the normal cell cycle activity. You can look at the attached papers for the inhibitors list.
Article Development of Cell Cycle Inhibitors for Cancer Therapy
Article Cell-cycle inhibitors: Three families united by a common cause
https://www.nature.com/articles/1207945/
Article CDK Inhibitors : Cell Cycle regulators and Beyond
Regards
Saurabh
You can use the similar approach that J.B. Gordon used to synchronize cell cycles while cloning xenopus, mild gamma or other LET radiation can cause cell cycle arrest.
- Badges
- Science topic
- Similar topics
- Biological Science
- Genetics
- Gene Expression