Hi, everyone, I am using pBR- recA promoter (Inducer: formaldehyde) with GFPuv construct in E.Coli. We want to detect the GFP signal in the presence of an inducer. We used different conc. of inducer in micromolar conc. in liquid and solid media. We are unable to get the gene induction. Please suggest me in this regard.
Thanks in advance
Michael J. Benedik
You might try a different inducer, mitomycin C is the commonly used to induce the recA promoter (or UV irradiation).
Also double check which E. coli strain you are using. Most typical lab cloning strains are recA mutants and you will not get induction in those strains. You need to be using a RecA+ strain, you will not get induction in recA- strains.
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