Hi. I would like to know if the absence of biological replicates can affect the qRT-PCR. I have some field samples of insects/mites in TriZOL Reagent and I plan have gene expression studies. Few of the samples collected from the field don't have biological replicate; meaning 1 vial from same day/time. SO:

Q1: Are the biological replicates necessary in gene expression studies?

Q2: If there is no biological replicate, can I increase number of technical replicates?

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