How do you design primers for microRNAs?

I have no prior knowledge of microRNAs and a member of my lab just started her project working on it. ive done primer designing before for my genes but it cant be applied to designing primers for...

23 November 2020 2,885 4 View

How do I save my HepG2 cell line?

I was given HepG2 cell line to culture and even after changing media and leaving it to attach, none of the cells have attached. I added a picture of my culture. I was told that this cell line has...

27 September 2020 4,357 6 View

Can any one suggest Tools for Calculating Graphlets degree distribution agreement.?

Hi all,I am analyzing Protein Protein interaction networks. I am using R studio for network analysis. For comparing real world networks with random network models there is a measure called...

04 May 2016 4,929 1 View

In an ballistic impact, for the same projectile with different velocities how the damage area on the front face varies with impact velocity?

In an ballistic impact, for the same projectile with different velocities, how the damage area on the front face varies with impact velocity. Projectile is 9 mm FMJ with lead core and brass jacket...

23 March 2016 8,403 2 View

How can I enter properties in Autodyn composite Modelling?

In AUTODYN modelling 11-direction represents the thickness direction. While entering orthotropic properties such as E11, E22 and E33, in what fashion we have to insert. Because E11 represents...

27 April 2015 384 2 View

Where can I find info on plasmids or processes for site directed mutation of C. glutamicum?

Can anyone help me in finding information about plasmids or processes that can be used for inserting specific gene sequences into coryneform bacterias, without leaving plasmid sequences into the...

13 January 2015 8,351 3 View

Can you give guidance to be a supplier of Oil Refineries for Mechanical components like Valve, Flanges etc. what is the road map?

11 November 2014 6,609 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View