What's the best way to analyse compositional data?

To give you a breif summary of my work, I am mostly looking at the percentage time a participant spends in a certain play state when compared to the entire observationn duration. I was using...

21 July 2019 4,618 2 View

CV vertex current is increasing and peak is shifting? Any good reference pointing those issues with dicussion?

Hello, I am looking for some good references that are mentioning/discussing/resoning the shifting of oxidation and reduction peaks as well as describing the reason of increasing the vertex current...

07 April 2019 6,969 3 View

Electrolysis using Ti electrode? better or bad?

Hello all, I am using a nano-indention device (JKR) to check adhesion capability of polymer gel under electric condition. I place two electrode on top of my sample. one electrode is indention stem...

18 March 2019 353 2 View

How to find structures of ligands for proteins?

I am looking for some ligands which bind to particular parts of a protein. Here by ligands, I am pointing to a database of all known ligand structures. Is there anything available like that?...

20 September 2018 1,931 6 View

Can I use uncapped RNA for over expression study?

RNA synthesized by MegaScript in vito transcription kit can be used for Rescue/over-expression study of gene? I know that, most eukaryotic mRNA molecules have a 5' 7-methyl guanosine residue or...

08 February 2018 360 2 View

How can I construct the structure of a protein which have multiple sub-units?

I want to construct the structure of a hetero dimer protein. How can I do that?

21 January 2018 9,876 4 View

What are mathematical calculations necessary to highlight a significant research on fashion , quality or other attributes of a garment?

To make a research significant to its followers mathematical analysis is required. What are those at the minimum label?

05 November 2017 3,796 4 View

How to measure PPM from the Concentration?

Hello, I am trying to measure Acetone exposure amount to the sensor. I used to blow air through acetone and pass the air into a flowmeter which gives reading in cc/Min. and then the output air...

11 January 2017 2,984 4 View

Raman Peak undefined?

24 July 2016 4,280 6 View

How can I improve His-B4GALNT1 purification using His Mag Excel Ni beads?

14 July 2016 3,544 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View