Hi , this is Qunting . I am working on the direction of single-cell library construction base on Tn5 transposase. Now I have a problem with my work , I sincerely ask for your help. My single cell method base on this paper " Tn5 transposase and tagmentation procedures for massively scaled sequencing projects", and have made little difference. My problem is success to construct single cell library , and unsteady on CNV detection. what else can influence CNV detection processes? Or what else can influence library construction in the one tube single cell?

I expecting your reply and help. Thank you!