MicroRNAs (miRNAs) are small, single chain,non-coding RNAs (ncRNAs) of approximately 17 to 24 nucleotides in length . At the cellular level, miRNAs regulate multiple normal
biological processes in immune system cells, including activation/inactivation, proliferation, differentiation, apoptosis, inflammation and autoimmunity, among others.
Studies clearly suggest there is a difference in miRNA expression in plasma & serum. Begin from sample collection, RNA isolation, cDNA synthesis, qPCR, spikes, and endogenous control can influence the expression. Furthermore, the sample's condition like normal and disease all matters. So until you do it will be very unpredictable.
Please find some cardio papers that discussed the difference between plasma and serum samples for miRNA expression.
Article Assessing sample and miRNA profile quality in serum and plas...
https://www.nature.com/articles/s41598-020-61507-z
Article Comparing the MicroRNA Spectrum between Serum and Plasma
Regards
Saurabh
hi
it depends on which miRNA you are looking for and type of your study and project
good luck
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