I would like to run southern blot, which lab provides this service? - FAQS.TIPS

Abdelhameed Elameen @Abdelhameed-Elameen

09 December 2022 1 6K Report

Hi,

I would like to run southern blot, which lab provides this service.

I have about 20 DNA samples with high quality and quantity, I just want to see the number of genes copies in these samples, these sample are transgenic materials.

Best regards

Elameen Dr. Abdelhameed Elameen

Researcher Biotechnology and Molecular Genetics

NIBIO, Norwegian Institute of Bioeconomy Research Pb 115, NO-1431 Ås M(+47) 90200875 www.nibio.no

Ali Ahmed Alawady

You can search in the field of specialization

Badges
Science topic

More Abdelhameed Elameen's questions See All

Available Solar Radiation Forecasting Dataset?

Dear RG members, My research team is searching for a real dataset for Solar Radiation Forecasting. If anyone can share us with a true dataset to test our new ML algorithms.

02 December 2020 2,173 36 View

Any available datasets for palm tree pests using thermal images?

We have a dataset about palm trees using thermal images. But, we are trying to find a public dataset to enhance our algorithms. Any help.

25 October 2020 9,997 33 View

May anyone help me to insert binary data into Popgene correctly?

Hello everyone, I am trying to use Popgene 32 software for analysis of binary data for dominant marker of five ISSR primers across five populations and having 38 loci. Unfortunately the program...

22 September 2020 7,369 4 View

May anyone recommend me a source for learning R programming language and R packages for biological research disciplines?

I want to learn R programming language but I do not know a source from which I can start, I need it for statistical analysis of Ecological, Molecular, floristic, and Taxonomic studies.

14 June 2020 4,985 9 View

Is it possible to use One trbulence model in fluid dynamics for all the practical applications ? if not, why?

There are many different turbulence models to be used in fluid dynamics applications. The question is why do we need to use different turbulence models for each application? is it possible to...

27 November 2018 5,238 24 View

Hello everybody, can anybody provide me with Erdetman method of acetolysis of pollen ?

I wonder if anybody can provide me with erdetman method of acetolysis of pollen grains for scanning electron microscopy, and the effective method for preservation of floral parts as long as possible?

07 April 2018 5,370 2 View

Does washing HeLa cells with water cause any problems to the cells? If so, Why?

I had to work with HeLa cells, and I have a question related to cell culture work. What would happen if I wash HeLa cells with water? I am asking this as when i wash the cells with water, all...

02 February 2017 9,843 4 View

What do you think about palm tree pests detection based on thermal images?

Can thermal images enhance the detection process of palm tree pests detection? Please share your opinions.

01 January 1970 5,844 7 View

Similar questions and discussions

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View