How are your experiences using FSH instead of PMSG for the superovulation of mice? Is the injection scheme the same as when using PMSG? Can the diluted solution be stored below -20 as it is the case for PMSG?
FRanziska
Hello everyone, I am working with Precision Cut liver slices of 250 µm. These need to be processed for FFPE. As you can imagenie they are very fragile and tend to break when I embedd them in...
16 November 2021 2,690 3 View
For most test statistics degrees of freedom are integers. For Welch's t-Test however, degrees of freedom are often decimals. According to APA 7, should I report the exact degrees of freedom or...
16 April 2021 5,802 1 View
Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...
11 January 2021 9,993 0 View
We aim to verify ATAC Seq data by CHIP-qPCR of the ROIs and are currently establishing all the controls. We use anti-H3K27me3 and H3K27Ac to pull down our histone marks of interest. We also used...
06 January 2021 6,662 0 View
Hi everyone, I am currently working on RNA extraction from bacterial (gram-neg) cultures for whole-transcritpome sequencing. I use a protocol as follow: Bacterial cell pellets are resuspended in...
11 November 2020 5,040 4 View
Hello, I am looking for an inducible episomal expression vector system which works in mammalian hematopoietic cells. The best scenario would be a bidirectional system, but an IRES-containing...
24 February 2020 8,116 1 View
Hi together, I am looking for an answer to an SPSS related question, using only ONE dummy coded IV with two groups. Other than that my model only includes moderators and control variables. After...
23 September 2019 2,667 5 View
I am running a VAR with a single stock price and a sentiment measure as endogenous variables, and the DAX, the IMX (real estate index), industry turnover, wages and unemployment as exogenous...
01 September 2019 2,748 12 View
I will be following up a cohort of participants who were last interviewed 10 years ago. At the time we used validated measure for all sorts of health, educational and social outcomes suitable for...
10 July 2019 9,506 11 View
Hi, I'm analyzing a CFA in MPLUS. To consider the multilevel structure of the data I'm using TYPE=COMPLEX. My supervisor told me to calculate the group-mean-centering of the items (before using...
17 June 2019 4,346 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View