Sensitivity analyze for crop evapotranspiration (ET0)?

Does anyone know how to perform a sensitivity analysis for crop evapotranspiration ( FAO Penman-Monteith equation) using daily meteorological data?

27 December 2020 9,552 3 View

How do I DERIVE equations for lumped elements (R, L, C) from S-parameters?

I have modeled the lumped element circuit for the 3D structure which has been simulated in HFSS. Now I need to derive the equations for the lumped elements keeping the S-parameters of the...

23 December 2020 172 3 View

No title

For academic writing.

17 December 2020 6,465 5 View

How to get nanocomposite of PEEK with Zirconia ?

Need a lengthy PEEK with Zirconia based nano composite of 180 mm length. Which method is possible? Tried injection molding no proper mix. Solvent casting process is also very costly. Kindly anyone...

13 December 2020 4,329 5 View

Choice of sampling time in discretization of control?

What I understand is while applying continuous control to a continuous-time system in MATLAB simulations, actually the control is being applied in discrete manner inherently due to the nature of...

28 November 2020 1,866 4 View

Simulation of sliding mode control for a maglev system?

I tried to simulate a maglev system with continuous time sliding mode but my simulation is showing error (there is a singularity at one of the integrators). I am attaching the modelling and...

16 November 2020 8,964 7 View

Can anyone suggest is there any way to compare the amount of chattering in 2 approaches of sliding mode control in quantitative way physically ?

Suppose 2 methods are shown to do sliding mode control of a given system. Please tell me if we can compare chattering in both by MATLAB or some other way

16 November 2020 3,251 3 View

On what basis two ceramics to be added in base metal to prepare hybrid metal matrix composites?

I am working on Mg-based metal matrix composites. I added two different types(A&B) with a suitable percentage(1%,2%,3% and 0.5%,1%,1.5%) of ceramics separately using DOE and tested. Now I want...

14 October 2020 2,984 9 View

Where i can get Ti based C94 catalyst ?

Ti based C94 catalyst is required for PBAT synthesis

12 September 2020 7,174 4 View

It is possible implement headonic regression model using apache spark milb?

My concern is to use Headonic regression model for market price index analysis.

10 September 2020 9,466 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View