Do you utilize Lipofectamine 3000 to transfect human splenic endothelial and fibroblast cell lines? If so, please share your transfection rate and the conditions under which you conduct the transfection.
Thanks,
Could you please share any information you might have about https://vaccine-congress.org/. I've been invited to present my research data at their conference, and I'd appreciate any input.
23 April 2024 7,103 1 View
Please share your experience with the journal.
11 February 2023 9,217 6 View
STZ is generally used to induce type 1 diabetes mellitus by intraperitoneal or intravenous injection at a dose of 45 to 65 mg/kg depending on the route of administration. It seems that...
19 August 2022 4,147 3 View
Hello, I am doing a research paper in which I will have to do semi-structured interviews to a group of SMEs from my hometown to analyze and define their needs and interests. Which tradition of...
27 January 2021 6,369 3 View
If so, where can I find it?
26 January 2018 8,320 1 View
I would like to know if shea butter can replace phospholipid
06 March 2017 9,913 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View