Hi,

We are checking the integrity of RNA isolated (using MagMAX 96 Total RNA Isolation kit) from mice tissue on a 1% agarose gel that contains a bit of bleach to get rid of RNases. Our 28S/18S ratios are less than 2 for most samples. Is this due to RNA degradation or is something wrong with our gel?

We sequenced some of our samples anyway using AmpliSeq RNA Transcriptome Mouse Gene Expression on the Ion Torrent S5. The amplicons that read end-to-end were around 13 000-14000 out of the total of 23 930 amplicons for all samples. Is this an indication of degradation or is this a normal value for RNA sequencing? We are wondering if we can continue sequencing the rest of our samples if we get similar results on the gels?

Thanks!