Other than RMSD, RMSF Radius ov gyration with significance
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
For timber production in forestry plantation, what is the best planting density for Juglans regia and J. nigra (Black walnut) for minimising lateral branch growth. In the UK forestry, this species...
21 July 2024 8,799 4 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View
What will be the pressure in Iron Iron-Carbide diagram? It is at atmospheric pressure?
05 July 2024 1,891 0 View
I need to learn decision table vailable in SWAT+. Please suggest some good papers or link for it.
19 June 2024 4,107 0 View
I am thinking to do the environmental flow assessment in a river basin, But i have only last 10 years of streamflow data. Please suggest if i can do some assessment regarding this?
19 May 2024 7,223 4 View
Actually I am looking for SWAT+ modeling help book/video to understand the basic processes happening in SWAT+ with great details.
13 May 2024 7,497 2 View
Performed the 1D photovoltaic simulation in COMSOL 5.4. While plotting IV curve, I got random graph as attached. What is the reason behind getting this kind of curve? I have attached a screenshot...
09 May 2024 1,204 0 View
sodium ion battery is not taking higher current when charging and also has a high voltage drop
06 May 2024 3,182 1 View
Borders are markers of nations. Do cartographical decisions involve emotions especially partitioning a unified territorial region ?
29 April 2024 3,715 5 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer
30 July 2024 3,354 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View