Other than RMSD, RMSF Radius ov gyration with significance
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Suggest me the method or some other software to calculate the above said data for nanoparticles.
21 February 2021 3,350 7 View
I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...
03 February 2021 2,187 3 View
Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...
10 January 2021 6,346 6 View
This is to design a protocol in which color obtained on nitrocellulose membrane can be converted or processed through any method to digits (numbers) to compare with different state of particular...
06 January 2021 9,309 1 View
Almost all the literature shows that the vertical distributions of soil organic carbon (SOC) & available nitrogen (N) is found decreasing with increased value of bulk density with respect to...
21 December 2020 670 10 View
I want to know if difference-in-difference approach can be used for two treatment groups. Can I use it it between them or should I first identify a control group and then compute the diff-in-diff...
20 December 2020 2,825 1 View
Sediment is relatively younger than the soil in the depositional environment as the sediments are consequence of the accretion of particles transported either by waters or by winds, whereas, soil...
12 December 2020 4,822 18 View
Dear researchers, I am currently trying to perform I-V measurements for thin films. Could anybody let me know what is the best way to perform I-V measurements using Keithley 2600. How to make...
01 December 2020 9,103 9 View
Bulk density of a forest soil sample in a community forest created under social forestry scheme in the district of Nadia, West Bengal is obtained as 0.81 gm/ cubic centimetre, comparatively too...
30 November 2020 4,706 33 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...
28 February 2021 298 3 View
I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...
27 February 2021 4,703 1 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
