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Questions related to Fluorimetry
Hi, I want to measure –(-)Epicatechin from cacao by fluorimetry. I have used acetone, water, acetic acid (70%, 29.5%, 0.5%) and sonication for the extraction. I have to let the sample evaporate...
08 August 2019 6,583 4 View
I'm studying a protein that degrades heme. I'm interested in using the active site's tryptophan fluorescence as a measure of binding affinity for heme, to obtain a Kd. Using an experiment in the...
12 December 2017 5,274 2 View
University of São Paulo School of Dentistry of Ribeirao Preto Department of Morphology, Physiology, and Basic Pathology Ribeirao Preto – São Paulo - Brazil The project entitled "Studies on the...
11 November 2017 8,048 1 View
I am currently checking ROS generation in human cells with DCFDA dye and measuring fluorescence on fluorimetry . What is the best way to express results? What calculations need to be done to...
04 April 2017 6,416 3 View
I am looking for an organ bath integrated in the fluorometer , allowing the simultaneous measurements of [Ca2+]c and tension in muscular strips . I found literature about CAF-100, Japan...
22 February 2017 476 2 View
02 February 2017 1,460 2 View
Good evening everybody, at present we are working on a sample of fluorine from the French Alps which displays two interesting phenomena: (1) Initially colorless and transparent, the samples turn...
01 January 2017 2,472 7 View
Experimental data was obtained from UV-vis and Fluorimetry techniques?
11 November 2016 7,955 2 View
I performed an assay using myeloma cell lines to understand the proteasome activity under normal and experimental conditions, using reversible proteasome inhibitor. And for such assay was...
08 August 2016 2,113 2 View
Dear researchers, I am working on cancer cell and human RBC cells.Testing cell viability,we using different assay like MTT and trypan blue.Now we have spectrofluorimeter.Can...
06 June 2016 5,121 1 View
I have trouble finding adequate method for fluorimetirc determination of saliva cortisol. I don't want to use ELISA nor RIA. Can you help me? Also, do I have to extract cortisol from saliva? How...
06 June 2016 9,028 3 View
I am looking to measure the co-factor NADPH produced by the cells, especially in the neutrophils after my treatment. Is there any particular colorimetric or fluorimetric assays are there to...
23 May 2016 8,184 1 View
05 May 2016 4,246 1 View
i am not able to understand bellow mentioned protocol, should i homogenize tissue then to florescence. The intracellular free Ca2+ level in thoracic aorta during contraction wasmeasured based on...
03 March 2016 638 2 View
Can anyone tell a standardised protocol for corticosterone estimation in rat serum?
02 February 2016 2,433 3 View
I currently use an absorbance-based ATPase assay centered around coupling released phosphates with the conversion of MESG by PNP. This results in increased product concentrations and a...
12 December 2015 999 3 View
02 December 2015 1,913 3 View
Which formula do we have to use in this calculation,either N1V1=N2V2 or something else?
11 November 2015 8,891 1 View
Hi everyone, I'm looking for black 6 or 12 well cell culture plates with clear bottoms for doing a fluorometric assay on my cells. I can easily find 96 well plates an even higher well number...
15 September 2015 2,618 3 View
09 September 2015 5,814 3 View
What is the main difference between dark or clear bottom plates to measure fluorescence?
09 September 2015 8,314 9 View
03 September 2015 2,933 8 View
Hello everyone, I am working with pericyte-like cells whic have long processes. I imagine Ca2+ in these cells using Oregon green and LSM800 laser scanning confocal micsroscopy. I add an agonist...
08 August 2015 1,582 3 View
I have an issue with high FAM fluorescence that lis the likely cause of incorrect genotype calls. I obtain twice as many FAM alleles relative to the reference genotypes of the control samples (64...
08 August 2015 2,078 2 View