Can you suggest me the protocol for XTT assay?

31 December 2014 4,813 5 View

Can anyone help me with cell isolation and culturing?

I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...

08 September 2014 6,431 20 View

Behavior asymmetry coefficient?

07 August 2014 2,682 10 View

Acetic acid induced writhings in mice?

I asked similar question some time ago and i want to try to get the help once more! During experiments with abdominal writhing induced with acetic acid very different results are obtained! In the...

07 August 2014 3,755 5 View

Does anyone have mouse brain atlas by Paxinos and Franklin (2001)?

06 July 2014 8,557 55 View

Has anyone had troubles with HID/AB staining?

After HID exposure, AB is binding or is bound much less than expected. The tissue in question is rat stomach.

04 May 2014 7,222 2 View

Can someone suggest method for determination of oxidised glutathione ?

But if it is possible spectrophotometric (not fluorescence)

04 May 2014 7,367 2 View

How to prepare a solution of silymarin suitable for i.p. application to rats?

What solvent, solution? What dose?

02 March 2014 2,410 4 View

Picrotoxin convulsions?

Could you suggest doses for induction of convulsions with picrotoxin?

11 December 2013 6,284 4 View

Did anyone have any troubles while performing acetic acid induced writhing test in mice?

Very different number of writhings among animals in group

11 December 2013 9,017 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View