Can you suggest me fluorimetric method for saliva cortisol determination?

More Nikola M Stojanovic's questions See All

Can you suggest me the protocol for XTT assay?

I have XTT powered substance (not kit!) so I have problem finding a proper protocol for applying this in macrophage/lymphocyte cell cultures. I have looked at many papers and kit protocols where...

31 December 2014 4,992 5 View

Can anyone help me with cell isolation and culturing?

I have some experience in cell isolation and culturing them. However those are mostly short assays (1-2h) and no real stuff. I want to know what culture medium is best for macrophages,...

08 September 2014 6,600 20 View

Behavior asymmetry coefficient?

These days I read in some article about behavior asymmetry coefficient that is calculated from parameters of open field test. However I didn't downloaded that paper. Now I can't find anything...

07 August 2014 2,855 10 View

Acetic acid induced writhings in mice?

I asked similar question some time ago and i want to try to get the help once more! During experiments with abdominal writhing induced with acetic acid very different results are obtained! In the...

07 August 2014 3,957 5 View

Does anyone have mouse brain atlas by Paxinos and Franklin (2001)?

Paxinos G, Franklin KBJ. 2001. The mouse brain in stereotaxic coordinates. San Diego, CA: Academic.

06 July 2014 8,904 55 View

Can someone suggest method for determination of oxidised glutathione ?

But if it is possible spectrophotometric (not fluorescence)

04 May 2014 7,535 2 View

Has anyone had troubles with HID/AB staining?

After HID exposure, AB is binding or is bound much less than expected. The tissue in question is rat stomach.

04 May 2014 7,402 2 View

How to prepare a solution of silymarin suitable for i.p. application to rats?

What solvent, solution? What dose?

02 March 2014 2,583 4 View

Picrotoxin convulsions?

Could you suggest doses for induction of convulsions with picrotoxin?

11 December 2013 6,452 4 View

Did anyone have any troubles while performing acetic acid induced writhing test in mice?

Very different number of writhings among animals in group

11 December 2013 9,198 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

Adam B Shapiro

Here are 4 different fluorescence-based cortisol assay methods:

http://www.ncbi.nlm.nih.gov/pubmed/16158300

http://www.cisbio.com/usa/drug-discovery/cortisol-assay

http://www.sciencedirect.com/science/article/pii/0039128X80900173

http://www.sciencedirect.com/science/article/pii/0009898179901190

Nikola M Stojanovic

Dear Adam,

thank you for links, however only first one is possibly suitable for my samples. However I'm not sure what should I do since it is for cell culture (in your paper) and I need it for SALIVA samples...

Bogdan Feliks Kania

After UCMV JU-AU Agricult Univ in Kakow it Elisa kit is better sensitive for cortisol detection, and I recommand to your investigation

bfk