Techniques using light resulting from PHYSICAL LUMINESCENCE emitted by LUMINESCENT PROTEINS and LUMINESCENT AGENTS. | Contact experts in Luminescent Measurements to get answers
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Questions related to Luminescent Measurements
Hello, I am using a lentivirus encoding CLuc to transduce target cells. I want to then lyse the cells in the presence of luciferin and measure luminescence. The kit which I had hoped to use...
16 September 2020 7,031 2 View
I need to know the best way to measure Human Chorionic Gonadotropin in culture supernatant and serum. Most of the literature use anelectro chemiluminescence assay. ELISA kits are available from...
08 August 2019 10,001 12 View
should be necessary in such rigid matrix? solvent could be CH2Cl2
02 February 2019 5,867 5 View
I have to do some bioluminescence assays, in that regard if anyone has a caspase-3 or any other caspase-luciferase construct, kindly let me know.
01 January 2019 4,474 3 View
Hello everyone, I have been using KRP as a media for PMNs during ROS assays (luminescence based detection of ROS generation upon infection of PMNs with Strep Pneumo). I couldn't find in the...
11 November 2018 7,386 2 View
I don't have any staurosporine, can I use something else instead?
10 October 2018 1,437 0 View
Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Thank you,
10 October 2018 6,588 0 View
Particularly, I want to know the cultured cell ATP assay Protocol, and data analysis (I am working on GBM cell line). Any prompt reply would be highly appreciated Thank you,
10 October 2018 2,518 0 View
Hello all, I wish to perform a luciferase luminescent measurement of ATP in E. coli. I have acquired a kit that requires less than 1 µM of KCl (and other monovalent metal salts). Also divalent...
19 April 2018 8,785 1 View
04 April 2018 7,357 1 View
I tried unsuccessfully to find any articles, where the Purcell enhancement of (Ge QD/Si-resonator) system would be shown from time-resolved experiments. Or any time-resolved measurements where Ge...
01 January 2018 8,182 0 View
Tuning luminescence of platinum complexes is well established by variation of cyclometalating ligand. The most common trend I find in literature is a red shift in the emission upon introducing...
09 June 2017 4,293 3 View
06 June 2017 4,574 3 View
Emission starts from within the excitation volume in the material. Looking from the top, this volume can be much bigger than the spatial extent of the point in the CL map for which the intensity...
26 April 2016 7,734 1 View
04 April 2016 3,798 1 View
We have spurious signal from empty sphere. We use sphere covered with Spectralon ( G8 or Quanta Phi). It seems that this sphere was contaminated. But we never used it before and it is brand new. I...
03 March 2016 4,527 5 View
I get vacuum UV photoluminescence specktums of rare earth doped inorganic compounds. when I check the literature to determine luminescence transitions, I came across emission spectras and...
02 February 2016 2,272 1 View
How can I convert lux into PPF (μmol m-2 s-1) for Red and Blue LEDs light?
01 January 2016 9,084 2 View
I am trying to measure change in cellular calcium ion concentration using aequorin expressing plant. I could find many research article and protocols about the measurement using the same system,...
09 September 2015 8,479 7 View
I working with Carbon nanodots(c-dots) I would like to measure the Quantum yield for prepared samples and also I am very eager to measure the Two Photon Absorption(TPA). In order to realize...
09 September 2015 7,463 7 View
I have faced similar problem in different spectrometers and hear a lot also about it from various researchers. Now I am wondering if any option can lit!
09 July 2015 4,703 2 View
07 July 2015 9,198 3 View
The Horiba DAS6 software in my computer is down. If you have the software in your lab, wound you mind helping me to fit the .das data into first order exponential decay and export the outcome to...
07 July 2015 7,226 10 View
i am interested to know about the metal complexes towards luminescence studies and calculations in best paper or review.
05 May 2015 3,669 0 View