Fluorimetry

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Questions related to Fluorimetry

What ratiometric method can be used to detect cytosolic calcium release after 12 hours of stimulation?

Hello guys, I am doing some experiments which involve increase of cytosolic calcium. At present i do not know what is the source of this increased calcium in cytosol. So i just wanted to confirm...

31 July 2015 8,944 3 View

I am looking for Microscopes/Optical Detection which allow the detection of Molecular Beacons on a Microfluidic platform?

I am planning on viewing several molecular beacons on a microfluidics systems and l was wondering if it is important to by an actual microscope or if systems exist that can be used for fluorimetry?

09 July 2015 8,230 10 View

I am looking for Microscopes/Optical Detection which allow the detection of Molecular Beacons on a Microfluidic platform?

I am planning on viewing several molecular beacons on a microfluidics systems and l was wondering if it is important to by an actual microscope or if systems exist that can be used for fluorimetry?

07 July 2015 8,152 11 View

What ratiometric method can be used to detect cytosolic calcium release after 12 hours of stimulation?

Hello guys, I am doing some experiments which involve increase of cytosolic calcium. At present i do not know what is the source of this increased calcium in cytosol. So i just wanted to confirm...

07 July 2015 3,072 4 View

Why did I obtain initial high fluorescence in Differential Scanning Fluorimetry?

I performed Differential scanning fluorimetry on BioRad CFX96 RT-PCR instrument. The protein concentration I used was in the range of 4-20mg/mL. I was getting initial high flourescence in the...

05 May 2015 7,604 10 View

Has anybody used a leaf fluorometer attached with a portable photosynthetic system-LI6400 XT?

I want to measure chlorophyll fluorescence in relation to the Cadmium stress in rice plants by using above mentioned leaf fluorometer. What is the correct way of doing this in order to take a...

02 February 2015 3,381 5 View

What is the proper concentration of SYPRO Orange and good buffer in Protein thermal shift assay using Real-time PCR system?

Hi! I am doing the Protein thermal shift assay to investigate the protein unfolding using StepOnePlus system - real-time PCR(qPCR) machine from Applied Biosystem, Life technology. My doubts and...

02 February 2015 558 19 View

What can I do if no Tm shift was observed in thermo shift assay when adding ATP analogues to ATPase protein?

Hi, I am doing thermo shift assay for one protein (It has an ATPase domain, thermostable, soluble) with 5mM to 15mM different ATP analogues(including ATP, ADP, ATP-gamma-S, etc) to see if I could...

21 January 2015 2,849 6 View

Could any one help me to do oxygen radical absorbance capacity (ORAC) assay for my sample?

I am working with a herbal plants and i wish to do ORAC assay for my samples to evaluate the antioxidant activity. Indeed, my problem is i don't know what is the suitable concentration should i...

08 January 2015 9,459 2 View

Which HT instrument should I for DSF with Bis-ANS dye?

Hi! I would like to run Differential Scanning Fluorimetry experiments with Bis-ANS as dye. Our AB-qPCR does not support the excitation/emission wavelenghts ( 395 nm/ 500 nm). Do you know of any HT...

07 January 2015 8,404 3 View

Which HT instrument should I for DSF with Bis-ANS dye?

Hi! I would like to run Differential Scanning Fluorimetry experiments with Bis-ANS as dye. Our AB-qPCR does not support the excitation/emission wavelenghts ( 395 nm/ 500 nm). Do you know of any HT...

01 January 2015 1,749 4 View

Could any one help me to do oxygen radical absorbance capacity (ORAC) assay for my sample?

I am working with a herbal plants and i wish to do ORAC assay for my samples to evaluate the antioxidant activity. Indeed, my problem is i don't know what is the suitable concentration should i...

01 January 2015 1,336 3 View

What can I do if no Tm shift was observed in thermo shift assay when adding ATP analogues to ATPase protein?

Hi, I am doing thermo shift assay for one protein (It has an ATPase domain, thermostable, soluble) with 5mM to 15mM different ATP analogues(including ATP, ADP, ATP-gamma-S, etc) to see if I could...

01 January 2015 4,154 7 View

Is it possible to compare two fluorimetry test with different bandwidths?

In fluorimetry tests with Pyrene probe (as fluorescent material), in first test the fluorescence emission was measured by 5 nm bandwidth, but in second test the emission was out of range and we...

11 November 2014 4,838 4 View

What are the slit and bandwidths in Fluorescence Spectra?

In Fluorimetry test, two parameters are reported: slit and bandwidth. What is these two parameters and how important are those parameters in fleorimetry test?

11 November 2014 8,093 5 View

What are the slit and bandwidths in Fluorescence Spectra?

In Fluorimetry test, two parameters are reported: slit and bandwidth. What is these two parameters and how important are those parameters in fleorimetry test?

04 November 2014 8,241 4 View

What is the best method for transformation of low amounts of large plasmids?

I received an shRNA plasmid of 7.5kb blotted onto a paper. The spot was cut out & dissolved in 200uL nuclease-free water and transformed 5uL into DH5-alpha competent cells, but found no...

08 August 2014 1,743 3 View

What is the best method for transformation of low amounts of large plasmids?

I received an shRNA plasmid of 7.5kb blotted onto a paper. The spot was cut out & dissolved in 200uL nuclease-free water and transformed 5uL into DH5-alpha competent cells, but found no...

04 August 2014 3,817 3 View

How can I use ethidium bromide to measure DNA concentration fluorimetry?

I want a protocol to measure DNA concentration fluorimetry using ethidium bromide dye.

06 June 2014 2,154 12 View

What is the correct procedure for uranium determination by laser fluorimetry?

This is very clearly demonstrated in every instrument manual. The procedure described by the author in his reply is absolutely wrong, quote " Five mL 5% sodium pyrophosphate (pH 7.0) solution was...

19 May 2014 1,602 10 View

What is the difference between static light scattering and 90 degree scattering?

I want clarification on the difference between static light scattering using Malvern zetasizer and 90 degree scattering using a spectrofluorimeter. Are both one and the same or completely...

05 May 2014 7,023 8 View

What is the correct procedure for uranium determination by laser fluorimetry?

This is very clearly demonstrated in every instrument manual. The procedure described by the author in his reply is absolutely wrong, quote " Five mL 5% sodium pyrophosphate (pH 7.0) solution was...

05 May 2014 6,576 11 View

Where can I find an article advising how to calculate the uncertainty in the assay for determination of chlorophyll "a" by fluorimetry?

see above

04 April 2014 9,039 0 View

What is the best solvent for measuring the fluorescent intensity of mesalamine?

We are planning to develop a fluorimetric method for the quantification of mesalamine and its forced degradation products. In that way, we are trying to optimize the solvent for the measurement of...

04 April 2014 2,296 6 View

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