How to expand the spheres?

Hello everyone, Greetings! I would like to know if we develop the spheres from secondary cell lines then,how can we expand those developed spheres in order to increase their number for further...

10 November 2019 5,441 2 View

Gene sequences for MACF1 isoforms?

Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...

09 October 2019 5,354 2 View

Designing of primers through uniprot?

Hello everyone, How can a researcher design the primers for a gene of interest using Uniprot.org.A researcher searches the gene of interest in Uniprot.org. in which he gets the gene name alongwith...

09 October 2019 5,607 3 View

How can we develop cancer stem cells from secondary cancer cell lines?

Hello All, I would like to know about the quickest methods as well as reagents to develop cancer stem cells from secondary cancer cell lines. Kindly attach the links for protocols if any. Thanking...

07 August 2019 6,890 3 View

How do the spheroids form in cancer cell lines using B27 supplement?

Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...

04 May 2019 1,680 3 View

Sarcosphere Isolation using centrifugation process?

Hello everyone, I would like to confirm whether we can isolate sarcospheres through centrifugation process or not. If yes, then how much should be the RPM or g, and for how much time....

03 April 2019 3,175 3 View

Is there someone who is working with curcumin currently?

03 April 2016 3,329 2 View

Can Curcumin stock be stored for 1 week treatment?

02 March 2016 7,203 4 View

Could someone tell me about different cell based bioassays we can perform using BT474 apart from HER2 ELISA and Cytostat?

01 January 1970 8,004 2 View

How stop solution i,e; 0.5M H2SO4 halts the reaction?

It is said that 0.5M H2SO4 acts as a stop solution to stop the reaction and turns the green color to yellow.I would like to know how it halts the reaction and how TMB and HRP play their role in...

01 January 1970 6,011 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

About loading of Doxorobicin on MOF (drug delivery)?

Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...

07 February 2021 748 2 View

Gain and offset values in Sentinel-2 L1C?

Hello everyone. I have been trying to apply radiometric calibration on Sentinel-2 L1C images (bands 2,3,4 and 8), but every time after layerstacking I come across this error: "Calibration...

28 January 2021 7,160 1 View

What is a good software for non-linear curve fitting rather than Excel and Lab Fit?

I am looking for good software for curve fitting. My curve equation is non-linear. I used Lab Fit and Excel software but could not find the curve equation with good accuracy. What software do you...

20 January 2021 5,554 13 View