How can we obtain a calibration curve at increasing nanomolar concentrations of Doxorubicin Hydrochloride, using fluorimetry?

11 November 2015 1 9K Report

Which formula do we have to use in this calculation,either N1V1=N2V2 or something else?

Suggestion: start by measuring the UV absorption spectra and fluorescence emission spectra of a doxorubicin solution (ex. 100 nM).

Some references:

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532009000800003

http://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm07452d#!divAbstract

Good work.

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