I am now carrying out anti diabetic assay in which performing enzyme inhibition assay. further i want to detect which type of enzyme inhibition assay is that. So can anyone suggest some protocol to follow to detect the same?
Inhibitory effect can be studied against Salivary alpha amylase and Pancreatic alpha amylase . Perform enzyme assays in presence and absence of inhibitor. Decline in enzyme activity is to be measured in presence of inhibitor.