I need a pharmacological protocol to induce acetylcholinesterase over-expression in mice brains, in order to use the brain homogenate as enzyme solution.
AcChE activity is high in mice brains. It is induced by organophospho inhibitors, but this is not useful due to the inactivation. It was reported that 17-b-estradiol is an inducer in young mice, but I have no experience on that. The reference is
Moudgil, V. K., & Kanungo, M. S. (1973). Effect of age of the rat on induction of acetylcholinesterase of the brain by 17β-estradiol. Biochimica et Biophysica Acta (BBA)-General Subjects, 329(2), 211-220.
Thank you Pr Solano For your interesting and helpfull answer
I have a question :
Acetylcholinesterase hydrolyzes acetylcholine to terminate cholinergic neurotransmission. Maybe the administration of acetylcholine can induce an over-expression of the enzyme as a physiological response ?
I have also conducted some research :
I Think pharmacological models of many disease can be helpful. For instance the streptozotocin model of diabetes (http://onlinelibrary.wiley.com/doi/10.1002/cbf.3014/full)
Also some peptides involved in alzheimer disease, however we do not possess those chemical (ihttp://www.sciencedirect.com/science/article/pii/S0168010202002018)
Some molecules that induces apoptosis such as MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, consequently it can increase AChE expression. (http://www.sciencedirect.com/science/article/pii/S1357272512003780)
Some flavonoids also has been shown to induce AChE activity in cell, but i think they will have also inhibitory activities. (http://www.sciencedirect.com/science/article/pii/S0009279716300965)
Of Course lesions can also induce over-expression, but i need a simple model (http://www.sciencedirect.com/science/article/pii/0006899372905331)
Here an interesting review about the enzymes http://www.nature.com/nrn/journal/v2/n4/abs/nrn0401_294a.html
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