Hello Everyone!

I am going to look PPO and POX enzyme activity in fruits. I am getting confuse to make a standard curve. Which reference substances should be taken while making a calibration curve for PPO and POX activity? 

I am using catechol as a substrate for PPO enzyme activity and guaiacol as a substrate for POX enzyme activity. I am following this article for the procedure. Or is there any other protocol ?

I want to calculate enzyme activity in the form of 1umol/min rather than on protein basis.

I am quite new in enzymology

Looking forward to your kind suggestions.

Thanking you in advance

Best Regards

Luna

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