Please dear god can somebody help me culture dendritic cells?

26 March 2025 1 4K Report

Does anybody have a tried and true method for generating dendritic cells from mouse bone marrow? I have tried about half a dozen protocols and I keep getting macrophages! And small cell counts as well. I've been at this for a month and a half and I am absolutely losing my mind.

Also, if you have a protocol that works for you, what are the flow cytometry markers you use to assess purity?

Louis-Charles Béland

Hello

In our paper (PMID: 38466819), we produce DC from BMDC, specifically cDC2. To produce cDC1, remove FLT3L from all the steps. Differenciation protocols will never be perfect and will result in a mixed cell population.

Markers you can check for purity are CD11b (pan monocyte marker), CD11c (pan DC marker), XCR1 (cDC1), CCR7 (cDC2), CD115 (mDC).

Good luck

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