Enzymes that add groups to 3' OH of nucleotides?

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Printing residues in an object in Pymol?

Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...

15 July 2024 1,727 1 View

Why is it that I keep on obtaining negative and positive %radical scavenging activity (RSA) in DPPH?

As a context, we have been optimizing the DPPH protocol for our seaweed ethanolic extract. We also use varying concentration points from the various methods in published journal articles, yet none...

28 May 2024 3,097 2 View

What are the best ways to integrate social studies education in an elementary school classroom?

Social studies education has been increasingly marginalized in elementary education in recent years and this has made an impact on students abilities to apply critical thinking skills to social...

27 February 2024 1,034 6 View

What are the antiserum titers required to successfully block rubella to measure measles titers in a combined MR vaccine?

Is there any available literature that describes, in depth, rubella antiserum titers that successfully block rubella when measuring measles potency via the TCID50 assay in a combination MR vaccine?

01 February 2024 6,018 1 View

Are there methods for quantitation of protein/peptide contamination in a sample that is primarily DNA?

We need to accurately measure the protein/peptide concentration in a sample that is at least 80% DNA (primarily ssDNA less than 80 bases long with some dsDNA also). A majority of the methods I've...

24 September 2023 9,156 3 View

Looking for International Validators?

Good day. I am looking for expert validators to check the suitability of our questionnaire in THE IMPACT OF CUSTOMER EXPERIENCE IN SOCIAL MEDIA MARKETING ON RELATIONSHIP QUALITY TOWARDS PURCHASE...

31 July 2023 6,874 0 View

Looking for Local Validators?

Good day. I am looking for expert validators to check the suitability of our questionnaire in THE IMPACT OF CUSTOMER EXPERIENCE IN SOCIAL MEDIA MARKETING ON RELATIONSHIP QUALITY TOWARDS PURCHASE...

02 June 2023 1,974 4 View

Are there cytokines that are specifically expressed in humans and not other organisms?

13 February 2023 5,814 2 View

How to make pair-wise comparisons from logistic regression mode output?

Hello, I have run a logistic model and I have four categories of a predictor variable. One option I have for comparing groups is comparing each group to every other possible group (like a Tukey's...

05 December 2022 4,309 1 View

Does the Promega antibody to HiBiT tag work for immunohistochemistry?

The Promega HiBiT monoclonal antibody (Clone 30E5) works very well for western blotting and immunofluorescence. I wonder if anyone has confirmed that it works well for immunohistochemistry of...

25 October 2022 2,624 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How does the catalase enzyme contribute to the process of photosynthesis in plants?

كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟

09 July 2024 7,512 1 View

What is the role of catalase enzyme in plants?

ماهو دور انزيم الكاتليز في النباتات؟

09 July 2024 5,824 2 View

What is the protocol for enzyme freeze drying?

what is the protocol for enzyme freeze drying?

09 July 2024 6,464 6 View