ELISA is an indirect method of diagnosis is it easy to to be used and replace direct microscopic examination Which may be need well trained experienced staff !!
ELISA is very easy to perform once you have a standardized protocol (optimal dilutions of your antibodies). In fact, ELISAs are often given to undergrad interns that have no lab skills yet, because it is easy but takes time.
Hello manar
I think your question can confuse us!
First, it is mandatory for each diagnostic laboratory to comply with the elementary standards of ethics and GLP. That includes quality control assurance, as well as completed technical training of the lab staff. On the other hand, ELISAs maybe does not be a very high complicate techniques, however requires some skill for its performance. For ex. some serological ELISAs for determining Ab vs Ag parasites shows cross reactivity, therefore, adequate dilutions are very important to avoid background therefore, so if the specialist does not have pipetting skill could make some impression.
Regards, Oscar
Dear Manar Ezz Elarab Ramadan
Please check this interesting study.
Article Laboratory Diagnosis of Parasites from the Gastrointestinal Tract
And i think the answer to your Q is 'YES' as the interpretation of the results needs experience.
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