Good afternoon,
Currently my team is performing animal studies with majority of the time using rats of different strains.
Our latest experiment was a weight loss study with diet-induced obesity SD rats, where now, as part of the analysis, we need to measure the following:
- HbA1c
- Insulin
- Adiponectin
- Leptin
- Ghrelin
- GLP-1
- Glucacon
Were have those samples as serum (except the HbA1c which is whole blood) and would be open to options when it comes to the analysis: different options for analysis and where to perform.
I've been having trouble in finding a company that can provide a service for the analysis of those above. I am aware one could purchase ELISA kits for those, but would prefer to outsource to a company, since the results of ELISA can vary greatly depending on the ability of the person performing it.
Thank you,
Larissa
Check this rated 5 online
https://www.mybiosource.com/hba1c-human-elisa-kits/glycated-hemoglobin-a1c/2031845
Not sure about RAT (renin angiotension?) but when measuring HbA1c in animals, note that the human methods that rely on beta N terminal glycation, including charge based alterations chromotographic mobility may not be appropriate. Immunoassays based on human haemoglobin glycation might also be suspect. Methods that generally measure overall haemoglobin glycation - ie affinity methods eg Beckman & Tosoh may be most appropriate.
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