What may be the reason to yield high absorbance value in negative control of MAC ELISA?

11 November 2019 1 6K Report

I am using IgM Mac ELISA for diagnosis of viral infection in our laboratory. I am strictly following the protocol as per the instruction from the manufacturer, but unfairly getting the high absorbance value in negative control. Have tried with all trouble shooting and even changed the kit batch number too. So can anyone help me out in rectifying the issue and solve it.

Kudi A. Audu

Interesting, but how does your positive controls behaves? I suggest you re-validate your ELISA apparatus and if possible, proceed on inter-laboratory testings as a form QA; compare outcomes.

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