How best to adhere bacteria to a microtiter plate?

04 April 2019 1 485 Report

I am looking to perform a whole cell ELISA with pertussis.

Hi Carmelina.

Coat the wells with poly-D-lysine, 50ul/well @ 0.1 mg/ml overnight at room temp. The following day, wash the wells 2x with either water or PBS and then air dry. You can store the plates in the fridge for several days.

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