Is it possible that the calculated LOQ is smaller than LOD in ELISA?

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Craig Silver

Your math is wrong, then. LOQ cannot be lower than LOD.

A. Asri

Craig Silver 80% of my ELISA data is non-detectable. I've tried establishing the LOD and LOQ by Excel Data Analysis using Regression. I can't pinpoint at which step did I mess up.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556583/

I use the same metrics for qPCR, LOB, LOD, and LOQ. On some situations you can have an LOD that is equivalent to an LOQ, but typically LOQ is higher because you have more faith in the data and validation data.

Craig Silver Thank you for the link. Btw, are you familiar with maximum likelihood estimation? I've tried using normal distribution but apparently it didn't fit my data. So I tried Weibull's distrib. However, my average value is negative so that too didn't work out. Is there any alternative distribution that I can use?