Can I do a thematic analysis of summary notes taken during workshops?

I ran a series of workshops in which experts were divided into small groups and asked to discuss various points. After the discussion, they returned to the larger group and reported their views...

03 October 2019 4,398 16 View

How do I analyse the relationship between number of self-referrals per year and the total population per same year, across 15 years?

I need to look at the relationship between the number of counselling self-referrals among students from an institution and the total number of students in the same institution, for each year...

04 November 2017 1,645 3 View

Graphene, graphene oxide and graphite d-spacing value and (hkl)

I would like to know what are the characteristic d-spacings and respective Miller indices for graphene, graphene oxide, reduced oxide graphene and graphite.

25 July 2017 5,399 9 View

Is MES a requirement to successfully cross-link a protein?

I want to cross-link collagen and from literature I found that the most common used buffer is MES and I do not have it yet. Is PBS a better option or distilled water? I know that EDC hydrolyses in...

04 March 2014 9,299 3 View

Testing the coefficients for pvcm in R?

I am working on modelling a panel using the panel variable coefficients model (pvcm) in R and I cannot figure out how to test these coefficients. With the "summary" command I get something like...

13 August 2013 4,210 2 View

Musicians' health literacy: Setting guidelines

01 January 1970 8,064 1 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Is there any free software for behaviour analysis of zebrafish ?

I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?

16 February 2021 1,541 1 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View