How do I validate my mutant zebrafish lines?

12 May 2022 1 9K Report

I am trying to establish new zebrafish knockouts as part of my project. I currently have F2s with a deletion that looks like it induces a premature stop codon. How can I validate that the zebrafish are indeed knockouts without specific antibodies?

Farheen Shafique

Have you tried to outcross them?

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Science method

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