I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the hippocampus. Here are the specifications of my experiment:
Strain: SD rats
Age: P30
Serotype: AAV9
Promoter: CMV
I'd like to know which ICV route (such as the 3rd ventricle or the 4th or any other) would be better to try to achieve the same. Please also advise on how much titer to inject if I want to achieve 4e10 GC at one localized area (say the motor cortex, for example). Thanks in advance.