When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

15 July 2024 0 2K Report

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65 degrees Celsius.

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