The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65 degrees Celsius.
Before entering a project, social license and consent from the community of the said area is very crucical as it determined whether the projects is accepted and viable to the community and...
14 July 2024 5,319 0 View
I'm evaluating the reproductive performance of quails. I have the fertility, and hatchability percentages for six different sire groups and would like to determine the significant difference...
14 July 2024 2,203 5 View
I have been trying to install PowerMarker on my Windows 10 PC but it is not working. One of the requirements for PowerMarker installation is MS NET Framework 1.1.4322 condition, however, Windows...
07 June 2024 1,553 1 View
In this presence age, AI is being used in many areas of human endeavour. Be that as it may, can human creativity, empathy, and complex problem-solving abilities be replaced with AI in any field?
04 June 2024 7,327 10 View
I am looking for for mentor in medicinal or organic chemistry to help guide me in my research and career development. I am also open to collaborating with researchers who share my interest. If...
26 May 2024 5,186 2 View
A researcher always have plainful, instead of joyful, experience when they reflecting of their action taken. I, as an autoethnographer, came across of this journey, and the result is...
19 May 2024 7,348 2 View
I cannot get the Raman spectrum for carbon based materials like graphene in Raman spectroscopy of 785 nm . What can be done for obtaining the proper Raman peaks for carbon based materials in 785...
15 May 2024 3,410 2 View
am working on a seminar topic
09 May 2024 9,788 0 View
Yes, we know the roles and responsibilities of the Holy Spirit, but I want to go a little deeper in my thesis or even research on the Holy Spirit, can anyone help me?
14 April 2024 9,636 8 View
In Uganda, the financial services sector is increasingly adopting artificial intelligence (AI) to streamline operations and enhance customer experiences. This research question seeks to explore...
10 April 2024 5,477 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am currently writing a systematic review
15 July 2024 9,176 1 View
Let me say I have a solar plant in operation and I want to build up a wind power plant, and later make a hybrid so that I could power the electrolyser as load for green hydrogen production.
14 July 2024 9,547 4 View
I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.
14 July 2024 1,892 3 View
What is a diamond open access journal and difference between green and gold access?
12 July 2024 9,717 3 View
How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...
10 July 2024 5,040 8 View