Hi,
Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely use for mouse splenocytes which gives good fresh and post-thaw cell viability (>70%).
Our mouse splenocytes are initially cultured and rested overnight in RPMI, 10% FBS, P/S, L-Glutamine and HEPES. For stimulation cells are resuspended in this media with the addition of IL-2 and Beta-mercaptoethanol and we have had no issues.
For hamster splenocytes however (using the same media and protocols for mouse splenocytes), we have been getting cell viabilities of around 50% which drops further after stimulating. Does a different culture media need to be used for hamster splenocytes? I found one paper that said RPMI was the best media, but haven't found any other publications that describe their isolation and culture of hamster splenocytes.
Any knowledge and tips on this would be gratefully received... Thanks!
Kind regards,
Lauren
Hi Lauren Maskell,
For hamster T cells, I would recommend X-Vivo media. Please find the reference attached, it worked for me.
Article Immune memory from SARS-CoV-2 infection in hamsters provides...
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs