I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on how to proceed with this, so looking for suggestion from mass spec and polymer chemistry experts on how to proceed?
Peptide mapping is a crucial technique used in the characterization of PEGylated proteins using mass spectrometry (MS)
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. PEGylation, the process of attaching polyethylene glycol (PEG) chains to proteins, is commonly employed to improve their pharmacokinetic and pharmacodynamic properties, the mass spectrometry fraternity handles peptide mapping of PEGylated proteins by employing analytical methods such as HPLC and mass spectrometry. Careful sample preparation and the use of advanced technologies contribute to the accurate characterization of PEGylation sites and the determination of structural information. These techniques play a crucial role in the development and characterization of PEGylated proteins for various biomedical applications.
It's important to note that the specific techniques and approaches used for peptide mapping of PEGylated proteins may vary depending on the research or analytical goals, the nature of the PEGylation, and the specific mass spectrometry instrumentation and protocols employed. Researchers in the mass spec fraternity may employ a combination of established methods and innovative approaches to handle the peptide mapping of PEGylated proteins.
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