How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is it possible to detect oxPAPC in the serum?

I would like to know if there is the possibility to detect oxidized phospholipids (oxPAPC) in the serum of mice. Is there an ELISA kit available?

10 January 2021 3,803 2 View

How to dissolve non-hydrolyzed lecithin in water?

Hello, Is it possible to dissolve non-hydrolyzed lecithin powder in water without oil content (or oil phase such as in emulsion). Is carbohydrate content or glucose content of non-hydrolyzed...

14 September 2020 535 4 View

How can we confirm biomolecule encapsualtion into liposome from UV-Vis or PL spectra?

I have been working with phospholipid vesicles and encapsulation of bio-molecules or drugs. But, i am unable to ensure whether it is taking place or not. what are the methods to do so including...

20 August 2020 7,076 7 View

How to separate phospholipids from betaine lipids using silica chromatography?

I tried separating betaine lipid from phospholipid and galactolipid from my 2D TLC plate but to no avail. Betaine lipid was found to overlap with some galactolipids under the condition of:- 1D:...

25 March 2020 641 3 View

Does anyone know the proper order of mixing "surfactant and lecithin" for liposome formulation?

For liposome formulation, I have to use a surfactant (Tween 80 or 20) beside the lecithin, but there isn't any specified order of mixing. someone add lecithin to the mixture of ethanol/surfactant;...

25 January 2020 6,754 1 View

What is the way to separate phospholipid from collagen peptide liposome to confirm EE%?

I am working on making liposome encapsulated collagen peptide. I'v tried to confirm EE%, checking concentration of only collagen peptide sample, which I made, yet I couldn't separate this collagen...

16 January 2020 6,387 3 View

Hi , can anyone tell me how we extract lecithin from egg?

for used as antioxidant

10 November 2019 498 3 View

If drug/lipid ratio increased in liposome, can encapsulation efficiency be affected?

Hi, I want to increase drug to lipid ratio when making liposome. I wonder if drug encapsulation efficiency (encapsulated drug/ total drug) can be affected by this ratio. For example, Comparing...

08 September 2019 6,203 6 View

If drug/lipid ratio increased in liposome, can encapsulation efficiency be affected?

Hi, I want to increase drug to lipid ratio when making liposome. I wonder if drug encapsulation efficiency (encapsulated drug/ total drug) can be affected by this ratio. For example, Comparing...

03 September 2019 8,415 7 View

Solubility of calcium ionophore II (ETH-129)?

Hello all - I am trying to add the calcium ionophore ETH-129 from Sigma ( https://www.sigmaaldrich.com/catalog/product/sial/21193?lang=en®ion=US ) to phospholipid bilayer/vesicles. The only...

03 September 2019 5,608 1 View