How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
25 July 2024 3,395 3 View
What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...
27 June 2024 2,138 2 View
in this link refractiveindex.info i didnt find i work on a simulation comsol of mesoporous perovskite i want to know the properties optoelectric and n,k coff
09 June 2024 2,071 4 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
11 May 2024 2,931 0 View
I had Titanium Dioxide powder & I need a specific answer for both points:- What is the most suitable solnavents used to convert TiO2 powder to TiO2 solution What is the most suitable methods...
30 April 2024 5,931 1 View
Hey, I am wondering which tests I can do to analyze my results and if there is a better way to analyze my results. If I have multiple treatments in columns and I have just one donor in a row,...
30 April 2024 335 2 View
Hey, Usually, when I perform total RNA extraction, I directly synthesize cDNA and run qPCR without even analyzing or testing the purity of RNA. I am asked to use 15µl of RNA when synthesizing...
20 March 2024 1,380 2 View
I have a human primary keratinocyte cell line, and I want to incubate them with 4 and 24h with Cathepsin S. However, I have gone through literature but I didn't find papers talking about Cathepsin...
12 March 2024 2,051 3 View
Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...
02 August 2024 6,980 4 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
16 July 2024 4,698 4 View
Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...
10 July 2024 4,083 3 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,671 6 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View