Why does exogenously applied iron salts cause leaves to become greener under sufficient iron conditions?

Do iron salts increase photosynthesis, or do they increase the size of chloroplasts, or cytochromes when iron is sufficient? In other words, is greening-up functional or structural under these...

26 January 2021 9,584 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do you calculate the substrate concentration of an assay in enyzme kinetics given the volume and the buffer volume at a given 6mM of the enyzme?

In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...

25 October 2020 1,702 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Trace metal speciation?

I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...

18 August 2020 4,333 4 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

What is the best way to hold AFM cantilevers during piranha cleaning?

Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...

24 March 2019 8,787 1 View

Is rice bran oil good for frying?

I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...

29 May 2018 1,311 1 View

Can somebody assist me by giving me standards for fumonisins for my MSC project?

I am a Master of Biotechnology student at Kenyatta University, working on a project on determination of Fumonisin FB1, FB1, FB3 and FB4 on maize samples. I would like to use HPLC technique of...

30 October 2017 2,079 3 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Does anyone know papers that use large databases of abundance of different groups of species?

03 October 2020 2,506 4 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

What are the limits of ACTIVE flight of various animals - Maximum Altitude (km) and Longest Continuous Travel Distance (km)?

Dear RG community, I am looking at the limits of ACTIVE flight of various animals. I am only interested in animals that are capable of active flight. Active flight (also called powered flight)...

28 May 2020 5,337 3 View

Can anyone recommend a hydrophone for recording amphibian calls underwater?

I have a relatively small budget but I would like to purchase a hydrophone that would enable me to call and describe the vocalisations of frogs underwater. The animals I hope to record are in...

06 January 2020 4,280 3 View

Does anyone have a key to aquatic macroinvertebrates from China ?

I will be working on stream amphibians in Sichuan in 2020, and part of the work will focus on the diet of some aquatic species and prey availability. I thought I'd identify macroinvertebrates with...

31 December 2019 6,315 6 View

How to euthanized humanely small reptile using alcoholic solution? Is this thing appropriate ?

How to euthanized small reptile using alcoholic solution? Some of the reference said that pentobarbital are good solution. However in field practice i have difficulties on availability and...

15 November 2019 1,375 6 View

How to euthanized humanely small reptile using alcoholic solution? Is this thing appropriate ?

How to euthanized small reptile using alcoholic solution? Some of the reference said that pentobarbital are good solution. However in field practice i have difficulties on availability and...

10 November 2019 9,341 6 View

Atypical colouration in caecilian amphibians?

I am curious to know about research publications discussing/reporting information on colour polymorphism in legless amphibians. Thanks in advance for your generous help. Venu

09 October 2019 444 0 View

Any suggestions for sample (tadpole liver) homogenization method before Ranavirus DNA extraction?

Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...

17 September 2019 8,404 6 View