Hello everyone,
I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating antibodies bound to the virus. Does anyone have a reliable protocol or references that could share for pre-treatment of infectious sample for isolation purposes?
Any guidance or suggestions would be greatly appreciated! Thank you in advance for your help.
Alireza Naderi Sohi
Dear Aldo
You can probably do this by lowering the pH using an appropriate buffer over a short period of time, or by a competitive approach via adding recombinant proteins similar to the surface proteins of the virus.
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