Why are my barium titanate sputtered thin films rapidly changing color when I take them out of the chamber?

Hello, I have been attempting to grow barium titanate thin films with sputtering by using 99.95% pure separate Barium and Titanium targets and varying oxygen flow rates to my sputtering chamber....

16 July 2020 9,297 4 View

How does sublimation temperature change in Ultra-High Vacuum?

We have a molecule that we are thinking of annealing/depositing onto a substrate in ultra-high vacuum (UHV). From our past experience, we know that the molecule sublimates around 90C at 10mtorr....

16 July 2019 1,784 4 View

What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

13 April 2019 3,255 2 View

Why is value of my blank higher than value of my samples in UV vis spectroscopy?

I am trying to plot a graph with R^2 > 0.8 with increasing values of Cadmium chelate from 0,2,4,6,8,10 mg/l . According to a paper I have read Cadmium-Alizarin Chelate is used in this method so...

16 February 2019 2,410 3 View

I have Cadmium +2 ions in a solution, What possible compounds can I add to this solution to make UV spectroscopy so as to find how much Cd +2 absorbs?

According to my teacher and research papers I have come to understand that UV spectroscopy requires Cadmium to be in a Chelating compound or a larger compound . What possible compounds can I add...

24 January 2019 4,711 2 View

Help Calculating Sample Size for Sickle Cell Disease Screening?

The current reported disease prevalence is .6% of the population. I've tried using the formula n=Z^2(p(1-p))/M^2 with a confidence level of 95% and M=0.05 but my n value is obviously too small if...

15 May 2018 5,253 3 View

Is there a specific statistical package(s) that would be best to run a longitudinal linear regression?

I am interesting in running a longitudinal linear regression and am wondering if there is anyone that has experience in this technique, and namely, if anyone can direct me to statistical packages...

06 August 2017 1,088 6 View

Does anyone have difficulty distinguishing BK viral cytopathic changes versus atypical urothelial cells in degenerated specimens?

This question pertains to urinary cytology specimens when screening for high grade urothelial carcinoma.

28 July 2015 4,684 2 View

How can I establish good 4T1 tumors in-vivo?

I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm...

04 June 2015 9,106 6 View

Does accelerated charge emit photons to all observers?

We know that a decelerated charge emits photons. So you can visualize an observer in a stationary frame of reference that detects these photons. However for another observer in an accelerated...

27 November 2014 2,929 28 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View